CD33 Protein (CD33) (AA 18-259) (His-Avi Tag)
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- 抗原 See all CD33 蛋白
- CD33
- 蛋白类型
- Recombinant
- 产品特性
- AA 18-259
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD33 protein is labelled with His-Avi Tag.
- 原理
- Human Siglec-3/CD33 Protein
- 序列
- Asp18-His259
- 产品特性
- Recombinant Human Siglec-3/CD33 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Asp18-His259.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human Siglec-3, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-Siglec-3 Antibody, hFc Tag with the EC50 of 5.1ng/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- Supplied as 0.22μm filtered solution in 20 mM PB, 500 mM NaCl ( pH 8.0).
- 储存条件
- -80 °C
- 储存方法
- Valid for 12 months from date of receipt when stored at -80°C., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD33
- 别名
- Siglec-3 (CD33 产品)
- 别名
- SIGLEC-3 Protein, SIGLEC3 Protein, p67 Protein, Siglec-3 Protein, gp67 Protein, CD33 molecule Protein, CD33 antigen Protein, CD33 Protein, Cd33 Protein
- 背景
- Sialic-acid-binding immunoglobulin-like lectin (Siglec) that plays a role in mediating cell-cell interactions and in maintaining immune cells in a resting state.They are sialoadhesin/CD169/Siglec-1, CD22/Siglec-2, CD33/Siglec-3, Myelin-Associated Glycoprotein (MAG/Siglec-4a) and Siglecs 5 to 11. To date, no Siglec has been shown to recognized any cell surface ligand other than sialic acids, suggesting that interactions with glycans containing this carbohydrate are important in mediating the biological functions of Siglecs.
- 分子量
- 29.6 kDa. Due to glycosylation, the protein migrates to 52-60 kDa based on Tris-Bis PAGE result.
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