CD3E & CD3G protein (His tag)
-
- 抗原
- CD3E & CD3G
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- His tag
- 序列
- Asp23-Glu120(CD3E) & Gln23-Gln106(CD3G)
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD3E&CD3G, His Tag at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 42.0ng/ml determined by ELISA. The affinity constant of 0.22nM as determined in SPR assay (Biacore T200). See testing image for detail.
-
CD3E & CD3G (AA 23-126) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G (AA 22-117) protein (Fc Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G protein (Fc-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD3E & CD3G (AA 22-117) protein (Fc Tag,Biotin)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G (AA 23-120) protein (His tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3E & CD3G (AA 23-108) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD3E & CD3G (AA 23-126) (Active) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3E & CD3G (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
CD3E & CD3G (AA 22-117) (Active) protein (His tag,AVI tag,DYKDDDDK Tag,Biotin)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3E & CD3G (AA 23-108) (Active) protein (His tag,DYKDDDDK Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
-
- 抗原
- CD3E & CD3G
- 别名
- CD3E&CD3G
- 背景
- CD3, CD3G, CD3g, T3G, CD3e, CD3E, CD3 epsilon&CD3 gamma, CD3E&CD3G,T-cell surface glycoprotein CD3 epsilon & CD3 gamma chain, also known as CD3E & CD3G , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
- 分子量
- 17.9 kDa (CD3E) and 17.9 kDa (CD3G). Due to glycosylation, the protein migrates to 25-40 kDa based on Tris-Bis PAGE result.
-
