TNFSF8
(Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
蛋白类型
Recombinant
产品特性
AA 63-234
宿主
人
资源
HEK-293 Cells
标记
This TNFSF8 protein is labelled with His tag.
原理
Human CD30 Ligand/TNFSF8 Protein
序列
Gln63-Asp234
产品特性
Recombinant Human CD30 Ligand/TNFSF8 Protein is expressed from HEK293 with His tag at the N-Terminus.It contains Gln63-Asp234.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CD30 Ligand, His Tag at 5μg/ml (100μl/well) on the plate. Dose response curve for Human CD30, hFc Tag with the EC50 of 9.9ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
TNFSF8
(Tumor Necrosis Factor (Ligand) Superfamily, Member 8 (TNFSF8))
CD153 Protein, CD30LG Protein, Cd30l Protein, CD30L Protein, TNFRSF8 Protein, TNFSF8 Protein, tumor necrosis factor (ligand) superfamily, member 8 Protein, TNF superfamily member 8 Protein, tumor necrosis factor superfamily member 8 Protein, Tnfsf8 Protein, TNFSF8 Protein
背景
CD30 ligand (CD30L)/TNFSF8 is a type II membrane protein belonging to the TNF superfamily. CD30L is expressed on the cell surface of activated T cells, B cells, and monocytes. The protein is also constitutively expressed on granulocytes and medullary thymic epithelial cells.CD30L is a cytokine that binds to TNFRSF8/CD30. Induces proliferation of T-cells.
分子量
20.7 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Tris-Bis PAGE result.