CD3D & CD3E protein (His tag)
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- 抗原
- CD3D & CD3E
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- His tag
- 序列
- Asp23-Glu120(CD3E) & Phe22-Ala105(CD3D)
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD3E&CD3D at 2μg/ml (100μl/Well) on the plate. Dose response curve for OKT3, mFc Tag with the EC50 of 38ng/ml determined by ELISA. The affinity constant of 0.36nM as determined in SPR assay (Biacore T200). See testing image for detail.
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CD3D & CD3E (AA 23-126) protein (Fc Tag)
宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 22-117) protein (Fc Tag)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E protein (Fc-Avi Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
CD3D & CD3E (AA 22-117) protein (Fc Tag,Biotin)宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-120) protein (His tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-108) protein (Fc Tag)宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
CD3D & CD3E (AA 23-126) (Active) protein (His tag)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (His tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (His tag,Fc Tag,DYKDDDDK Tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
CD3D & CD3E (AA 23-126) (Active) protein (Fc Tag,His tag,DYKDDDDK Tag,AVI tag,Biotin)宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD3D & CD3E
- 别名
- CD3E&CD3D
- 别名
- AI504783 Protein, CD3 Protein, CD3epsilon Protein, T3e Protein, CD3 antigen, epsilon polypeptide Protein, Cd3e Protein
- 背景
- CD3, CD3e, CD3E, CD3d, T3D, CD3D, CD3E&CD3D, CD3 delta&CD3 epsilon,T-cell surface glycoprotein CD3 epsilon & CD3 delta chain, also known as CD3E & CD3D , are single-pass type I membrane proteins.When antigen presenting cells (APCs) activate T-cell receptor (TCR), TCR-mediated signals are transmitted across the cell membrane by the CD3 chains CD3D, CD3E, CD3G and CD3Z. All CD3 chains contain immunoreceptor tyrosine-based activation motifs (ITAMs) in their cytoplasmic domain.
- 分子量
- 17.9 kDa (CD3E) and 16.7 kDa (CD3D). Due to glycosylation, the protein migrates to 35-42 kDa and 25 kDa based on Tris-Bis PAGE result.
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