CD200 Protein (CD200) (His tag)
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- 抗原 See all CD200 蛋白
- CD200
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This CD200 protein is labelled with His tag.
- 序列
- Gln31-Gly232
- 纯度
- > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human CD200 at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Human CD200 R1, hFc Tag with the EC50 18ng/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge tubes before opening. Reconstituting to a concentration more than 100 μg/mL is recommended (usually we use 1 mg/mL solution for lyophilization). Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 5 % trehalose is added as protectant before lyophilization.
- 储存条件
- 4 °C,-80 °C
- 储存方法
- Reconstituted protein stable at -80°C for 12 months, 4°C for 1 week. Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- CD200
- 别名
- CD200 (CD200 产品)
- 背景
- CD200, MOX1, MOX2, My033, MRC, OX-2, OX2,CD200 and its receptors are highly expressed in the lung, on epithelial cells and leukocytes, and emerging evidence links dysregulation of the CD200 pathway with asthma. Moreover, pharmacological modulation of CD200 receptors was shown to improve clinical and inflammatory outcomes of preclinical asthma models.
- 分子量
- 23.5 kDa. Due to glycosylation, the protein migrates to 40-60 kDa based on Tris-Bis PAGE result.
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