This CCL5 protein is labelled with His tag,SUMO Tag.
原理
Human CCL5 Protein
序列
Ser24-Ser91
产品特性
Recombinant Human CCL5 Protein is expressed from E.coli with His-SUMO tag at the N-Terminus.It contains Ser24-Ser91.
纯度
> 95 % as determined by Tris-Bis PAGE
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Human CCL5, His Tag at 0.5μg/ml (100μl/Well) on the plate. Dose response curve for Anti-CCL5 Antibody, hFc Tag with the EC50 of 8ng/ml determined by ELISA. See testing image for detail.
CCL5
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
> 95 % as determined by reducing SDS-PAGE.
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in 20 mM Tris,0.3M NaCl ( pH 8.5). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
The CCR5 and the CCL5 ligand have been detected in some hematological malignancies, lymphomas, and a great number of solid tumors, but extensive studies on the role of the CCL5/CCR axis were performed only in a limited number of cancers.
分子量
21 kDa. Due to the protein can cleaved into two chains, so it migrates to 15-18 kDa and 22-25 kDa based on Tris-Bis PAGE result.