BTN3A1
(Butyrophilin, Subfamily 3, Member A1 (BTN3A1))
蛋白类型
Recombinant
产品特性
AA 30-254
宿主
人
资源
HEK-293 Cells
标记
This BTN3A1 protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Human BTN3A1/CD277 Protein
序列
Gln30-Gly254
产品特性
Recombinant Biotinylated Human BTN3A1/CD277 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Gln30-Gly254.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Anti-BTN3A1 Antibody, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Human BTN3A1, His Tag with the EC50 of 15.4ng/ml determined by ELISA. See testing image for detail.
BTN3A1
宿主: Cynomolgus
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Crystallography grade
BTN3A1
宿主: 人
宿主: 大肠杆菌(E. Coli)
Recombinant
>95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot.
SDS, WB, ELISA, Crys
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
BTN3A1
(Butyrophilin, Subfamily 3, Member A1 (BTN3A1))
BT3.1 Protein, BTF5 Protein, CD277 Protein, BTN3A1 Protein, butyrophilin subfamily 3 member A1 Protein, BTN3A1 Protein, LOC100447925 Protein
背景
The three butyrophilin BTN3A molecules, BTN3A1, BTN3A2, and BTN3A3, are members of the B7/butyrophilin-like group of Ig superfamily receptors, which modulate the function of T cells. BTN3A1 controls activation of human Vγ9/Vδ2 T cells by direct or indirect presentation of self and nonself phosphoantigens (pAg).
分子量
27.1 kDa. Due to glycosylation, the protein migrates to 30-35 kDa based on Tris-Bis PAGE result.