BCMA (TNFRSF17)
(Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))
蛋白类型
Recombinant
产品特性
AA 1-49
宿主
小鼠
资源
HEK-293 Cells
标记
This BCMA protein is labelled with His-Avi Tag,Biotin.
原理
Biotinylated Mouse BCMA/TNFRSF17 Protein
序列
Met1-Thr49
产品特性
Recombinant Biotinylated Mouse BCMA/TNFRSF17 Protein is expressed from HEK293 with His tag and Avi tag at the C-Terminus.It contains Met1-Thr49.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse APRIL, hFc Tag at 2μg/ml (100μl/well) on the plate. Dose response curve for Biotinylated Mouse BCMA, His Tag with the EC50 of 2.6ng/ml determined by ELISA. The affinity constant of 5.64 μM as determined in SPR assay (Biacore T200). See testing image for detail.
TNFRSF17
宿主: 人
宿主: HEK-293 Cells
Recombinant
The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
ELISA
TNFRSF17
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
Active
限制
仅限研究用
状态
Lyophilized
溶解方式
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
BCMA (TNFRSF17)
(Tumor Necrosis Factor Receptor Superfamily, Member 17 (TNFRSF17))
B-cell maturation antigen (BCMA or BCM), also known as tumor necrosis factor receptor superfamily member 17 (TNFRSF17), is a protein that in humans is encoded by the TNFRSF17 gene.TNFRSF17 is a cell surface receptor of the TNF receptor superfamily which recognizes B-cell activating factor (BAFF).
分子量
8.5 kDa. Due to glycosylation, the protein migrates to 12-16 kDa based on Tris-Bis PAGE result.