Recombinant Mouse ARTN Protein is expressed from HEK293 with hFc tag at the N-Terminus.It contains Ala112-Gly224.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Mouse GFRA3, His Tag at 5μg/ml (100μl/Well) on the plate. Dose response curve for Mouse ARTN, hFc Tag with the EC50 of 0.12μg/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in 50 mM Glycine,150 mM NaCl ( pH 3.2).
缓冲液
Lyophilized from 0.22μm filtered solution in 50 mM Glycine,150 mM NaCl ( pH 3.2). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
ARTN Protein, ENOVIN Protein, EVN Protein, NBN Protein, neublastin Protein, artemin Protein, ARTN Protein, Artn Protein
背景
Artemin (ARTN) is a member of glial cell line-derived neurotrophic factor (GDNF) family of ligands, and its signaling is mediated via a multi-component receptor complex including the glycosylphosphatidylinositol-anchored GDNF family receptors a (GFRa1, GFRa3) and RET receptor tyrosine kinase. The major mechanism of ARTN action is via binding to a non-signaling co-receptor. The major function of ARTN is to drive the molecule to induce migration and axonal projection from sympathetic neurons.
分子量
39.4 kDa. Due to glycosylation, the protein migrates to 65-80 kDa based on Tris-Bis PAGE result.