APOH
(Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))
蛋白类型
Recombinant
产品特性
AA 20-345
宿主
Cynomolgus
资源
HEK-293 Cells
标记
This APOH protein is labelled with His tag.
原理
Cynomolgus APOH Protein
序列
Gly20-Cys345
产品特性
Recombinant Cynomolgus APOH Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Gly20-Cys345.
纯度
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
过滤
0.22 μm filtered
内毒素水平
Less than 1EU per μg by the LAL method.
Biological Activity Comment
Immobilized Cynomolgus APOH, His Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Anti-APOH Antibody, hFc Tag with the EC50 of 97.2ng/ml determined by ELISA. See testing image for detail.
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
缓冲液
Lyophilized from 0.22μm filtered solution in PBS, 100 mM Arginine ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
储存条件
-20 °C,-80 °C
储存方法
-20 to -80°C for 12 months as supplied from date of receipt.,-80°C for 3-6 months after reconstitution.,2-8°C for 2-7 days after reconstitution.,Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
有效期
12 months
抗原
APOH
(Apolipoprotein H (Beta-2-Glycoprotein I) (APOH))
Apolipoprotein (apo)H (also known as beta 2 glycoprotein-I) is a glycoprotein synthesized by liver cells and it is present in the blood associated with plasma lipoproteins. APOH displays a genetically determined structural polymorphism: three alleles (APOH*1, APOH*2, APOH*3) at a single locus on chromosome 17 code for different isoforms, and population studies have shown that APOH*2 is the most frequent allele.
分子量
37.39 kDa. Due to glycosylation, the protein migrates to 50-65 kDa based on Tris-Bis PAGE result.