2B4 Protein (AA 22-221) (His tag)
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- 抗原 See all 2B4 (CD244) 蛋白
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 蛋白类型
- Recombinant
- 产品特性
- AA 22-221
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This 2B4 protein is labelled with His tag.
- 原理
- Human 2B4/CD244/SLAMF4 Protein
- 序列
- Cys22-Arg221
- 产品特性
- Recombinant Human 2B4/CD244/SLAMF4 Protein is expressed from HEK293 with His tag at the C-Terminus.It contains Cys22-Arg221.
- 纯度
- > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
- 过滤
- 0.22 μm filtered
- 内毒素水平
- Less than 1EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized Human 2B4, His Tag at 1μg/ml (100μl/Well) on the plate. Dose response curve for Human CD48, hFc Tag with the EC50 of 0.2μg/ml determined by ELISA. See testing image for detail.
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/mL is recommended. Dissolve the lyophilized protein in distilled water.
- 缓冲液
- Lyophilized from 0.22μm filtered solution in PBS ( pH 7.4). Normally 8 % trehalose is added as protectant before lyophilization.
- 储存条件
- -20 °C,-80 °C
- 储存方法
- -20 to -80°C for 12 months as supplied from date of receipt., -80°C for 3-6 months after reconstitution., 2-8°C for 2-7 days after reconstitution., Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
- 有效期
- 12 months
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- 抗原
- 2B4 (CD244) (Natural Killer Cell Receptor 2B4 (CD244))
- 别名
- 2B4 (CD244 产品)
- 别名
- CD244 Protein, 2B4 Protein, NAIL Protein, NKR2B4 Protein, Nmrk Protein, SLAMF4 Protein, C9.1 Protein, F730046O15Rik Protein, Ly90 Protein, CD244 molecule Protein, CD244 natural killer cell receptor 2B4 Protein, CD244 Protein, Cd244 Protein
- 背景
- 2B4 (CD244) is expressed by memory-phenotype CD8 T cells and all natural killer (NK) cells. The ligand for 2B4, CD48, is expressed on hematopoietic cells. 2B4 is conserved in humans and mice, and a number of reports have linked 2B4 with activation of lymphocytes.Engagement of 2B4 on NK cell surfaces with specific antibodies or CD48 can trigger cell mediated cytotoxicity, interferon γ secretion, phosphoinositol turnover and NK cell invasiveness.
- 分子量
- 23.1 kDa. Due to glycosylation, the protein migrates to 45-70 kDa based on Tris-Bis PAGE result.
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