HLA-B*35:01 protein (SBP Tag)
宿主: 人
宿主: Mammalian Cells
Recombinant
> 80 %
BP, CP, ELISA, Neut
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- 抗原
- HLA-B*35:01
- 蛋白类型
- Recombinant
- 宿主
- 人
- 资源
- Mammalian Cells
- 标记
- SBP Tag
- 应用范围
- Blocking Peptide (BP), Control Peptide (CP), ELISA, Neutralization (Neut)
- 原理
- The product is HLA-B*35:01 complexed with β2-microglobulin (b2m)
- 序列
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heavy chain: GSHSMRYFYT AMSRPGRGEP RFIAVGYVDD TQFVRFDSDA ASPRTEPRAP WIEQEGPEYW DRNTQIFKTN TQTYRESLRN LRGYYNQSEA GSHIIQRMYG CDLGPDGRLL RGHDQSAYDG KDYIALNEDL SSWTAADTAA QITQRKWEAA RVAEQLRAYL EGLCVEWLRR YLENGKETLQ RADPPKTHVT HHPVSDHEAT LRCWALGFYP AEITLTWQRD GEDQTQDTEL VETRPAGDRT FQKWAAVVVP SGEEQRYTCH VQHEGLPKPL TLRWEPSSQS TIPI
light chain: IQRTPKIQVY SRHPAENGKS NFLNCYVSGF HPSDIEVDLL KNGERIEKVE HSDLSFSKDW SFYLLYYTEF TPTEKDEYAC RVNHVTLSQP KIVKWDRDM
Sequences without tags. Tag location is at the discretion of the manufacturer. - 特异性
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HLA-B*35:01 complexed with b2m, P01889 / P61769
HLA-B35 is frequently found in the Caucasoid and the Amerindian major ethnic group, but also in Orientals and Blacks. HLA-B*35:01 is recognized by anti-Bw6 antibodies. - 产品特性
- HLAs are produced in mammalian cell cultures as recombinant, truncated, soluble antigens, in complex with β2-microglobulin (HLA class I). As part of our quality control, all HLA are analyzed for purity by gel electrophoresis, concentration is determined by ELISA (0.5 mg/ml) and their functionality is verified with a specific antiserum.
- 纯化方法
- Chromatography
- 纯度
- > 80 %
- 内毒素水平
- Endotoxins have not been removed
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- 应用备注
- ELISA: 1 - 100 ng/mL
- 说明
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sandwich ELISA, direct ELISA, bead ELISA, reactive with antibodies W6/32 and BBM.1 and specific antiserum, B cell ELI Spot (predicted), in vitro memory B cell stimulation (predicted), HLA competition assay (predicted), control in various assays (predicted)
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.5 mg/mL
- 缓冲液
- TRIS based buffer, pH 8.0, Sodium azide
- 储存液
- Sodium azide
- 注意事项
- This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- 储存条件
- 4 °C
- 有效期
- 6 months
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- 抗原
- HLA-B*35:01
- 背景
- Human Leukozyte Antigen, human Antigen-presenting major histocompatibility complex class I (MHCI) molecule. Expressed on all cells, displays peptides to CD8-positive T cells. Involved in cellular immune response.
- 分子量
- approx. 50 kDa (38 kDa heavy chain + 12 kDa b2m)
- UniProt
- P01889
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