IDH1 Protein (Arg132Cys-Mutant) (His tag)
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- 抗原 See all IDH1 蛋白
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- 蛋白类型
- Recombinant
- 产品特性
- Arg132Cys-Mutant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This IDH1 protein is labelled with His tag.
- 原理
- Human IDH1 (R132C) Protein, His Tag (MALS verified)
- 序列
- Met 1 - Leu 414
- 产品特性
- Human IDH1 (R132C), His Tag (ID1-H51H8) is expressed from E.coli cells. It contains AA Met 1 - Leu 414 (Accession # O75874-1(R132C).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
- Top Product
- Discover our top product IDH1 蛋白
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Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 2-414) protein (His tag)
IDH1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by Tris-Bis PAGE,> 90 % as determined by HPLC
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 341-414) protein (His-IF2DI Tag)IDH1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) protein (His tag)IDH1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1) (AA 1-414) protein (GST tag)IDH1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
- This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 48.5 kDa. The protein migrates as 47-50 kDa under reducing (R) condition due to glycosylation.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 缓冲液
- 50 mM Tris, 150 mM NaCl, 1 mM TCEP, pH 7.5
- 储存条件
- -80 °C
- 储存方法
- -70°C
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- 抗原
- IDH1 (Isocitrate Dehydrogenase 1 (NADP+), Soluble (IDH1))
- 别名
- IDH1 (IDH1 产品)
- 别名
- IDCD Protein, IDH Protein, IDP Protein, IDPC Protein, PICD Protein, NADP-CICDH Protein, AI314845 Protein, AI788952 Protein, E030024J03Rik Protein, Id-1 Protein, Idh-1 Protein, Idpc Protein, cb876 Protein, fm90e09 Protein, im:7143416 Protein, wu:fm90e09 Protein, F23E12.180 Protein, F23E12_180 Protein, IDH-I Protein, NAD+ DEPENDENT ISOCITRATE DEHYDROGENASE SUBUNIT 1 Protein, isocitrate dehydrogenase 1 Protein, isocitrate dehydrogenase I Protein, isocitrate dehydrogenase (NADP(+)) 1, cytosolic Protein, isocitrate dehydrogenase 1 (NADP+), soluble Protein, isocitrate dehydrogenase 1 (NADP+) L homeolog Protein, isocitrate dehydrogenase 1 Protein, IDH1 Protein, Idh1 Protein, idh1.L Protein, idh1 Protein
- 背景
- Synonyms:IDH1,PICD,IDP,Description:The IDH enzymes catalyze the oxidative decarboxylation of isocitrate to alpha-ketoglutarate (α-KG), producing nicotinamide adenine dinucleotide phosphate (NADPH) in the process via the citric acid cycle. Eukaryotic cells express two distinct classes of IDHs that utilize either NAD or NADP as their cofactors and serve diverse biological functions. NAD-dependent IDH is localized to the mitochondrial matrix and is well known for its central role for energy production in the Krebs cycle. NADP-dependent IDHs are primarily located either in mitochondria or cytoplasm . Each NADP-dependent isozyme is a homodimer. Mutations of Arg132 of human IDH1 result in a reduced ability of the enzyme to convert isocitrate to alpha -ketoglutarate, but the enzyme acquires the ability to generate 2-hydroxyglutarate (2HG) from alpha-ketoglutarate,2-HG is elevated in several tumor types, including a subset of AMLs.
- 分子量
- 48.5 kDa
- 途径
- Warburg Effect
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