The in vivo grade recombinant rabbit IgG Fc protein (rIgG Fc, Cys 105 - Lys 323 of the constant region of the IgG heavy chain, Accession # P01870-1) was produced in mammalian cells and purified by protein A. The rIgG Fc protein includes the CH2 (114-213) and CH3 (222-323) domains of the heavy chain constant region as well as the hinge region (105-113) before the CH2 domain.
宿主: 人
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
WB
应用备注
The recombinant rabbit IgG Fc protein and mutants can be used as the isotype control of the rIgG antibodies and the rIgG Fc fusion proteins in various in vitro and in vivo experiments.
限制
仅限研究用
状态
Liquid
浓度
1 mg/mL
缓冲液
PBS, pH 7.4
储存条件
-20 °C
储存方法
1 month from date of receipt, 2 to 8°C as supplied. 3 months from date of receipt, -20°C to -70°C as supplied.
有效期
3 months
抗原
IgG Fc
分子量
Predicted 24.8 kDa. Due to glycosylation, the recombinant rabbit IgG Fc protein migrates to 28-32 kDa based on the Bis-Tris PAGE result.