RSV Fusion Protein Protein (His tag)
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- 抗原 See all RSV Fusion Protein (RSV F) products
- RSV Fusion Protein (RSV F) (Respiratory Syncytial Virus Fusion Protein (RSV F))
- 蛋白类型
- Recombinant
- 宿主
- Respiratory Syncytial Virus (RSV)
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资源
- Baculovirus infected Insect Cells
- 标记
- This RSV Fusion Protein protein is labelled with His tag.
- 原理
- Recombinant RSV Fusion protein / RSV-F (Strain RSS-2) Protein (His Tag)
- 序列
- Met 1-Thr 529
- 产品特性
- A DNA sequence encoding the extracellular domain of human RSV (strain RSS-2) fusion protein (P11209) (Met 1-Thr 529) was expressed with a polyhistidine tag at the C-terminus.
- 纯度
- > 95 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method
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- 说明
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63 kDa and 44-53 KDa
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % gly Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the printed manual.
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- RSV Fusion Protein (RSV F) (Respiratory Syncytial Virus Fusion Protein (RSV F))
- 别名
- RSV Fusion Protein (RSV F 产品)
- 背景
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Background: Human respiratory syncytial virus (HRSV) is the most common etiological agent of acute lower respiratory tract disease in infants and can cause repeated infections throughout life. It is classified within the genus pneumovirus of the family paramyxoviridae. Like other members of the family, HRSV has two major surface glycoproteins (G and F) that play important roles in the initial stages of the infectious cycle. The G protein mediates attachment of the virus to cell surface receptors, while the F protein promotes fusion of the viral and cellular membranes, allowing entry of the virus ribonucleoprotein into the cell cytoplasm. The fusion (F) protein of RSV is synthesized as a nonfusogenic precursor protein (F), which during its migration to the cell surface is activated by cleavage into the disulfide-linked F1 and F2 subunits. This fusion is pH independent and occurs directly at the outer cell membrane, and the F2 subunit was identifed as the major determinant of RSV host cell specificity. The trimer of F1-F2 interacts with glycoprotein G at the virion surface. Upon binding of G to heparan sulfate, the hydrophobic fusion peptide is unmasked and induces the fusion between host cell and virion membranes. Notably, RSV fusion protein is unique in that it is able to interact directly with heparan sulfate and therefore is sufficient for virus infection. Furthermore, the fusion protein is also able to trigger p53-dependent apoptosis.
Synonym: F Protein, RSV, HRSVgp08 Protein, RSV, Human respiratory syncytial virus
- 分子量
- 57.8kDa
- UniProt
- P11209
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