GALE Protein (His tag)
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- 抗原 See all GALE 蛋白
- GALE (UDP-Galactose-4-Epimerase (GALE))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This GALE protein is labelled with His tag.
- 原理
- Recombinant Human GALE Protein (His Tag)
- 序列
- Met 1-Ala348
- 产品特性
- Recombinant Human UDP-Glucose 4-Epimerase is produced by our E.coli expression system and the target gene encoding Met1-Ala348 is expressed with a 6His tag at the N-terminus.
- 纯度
- > 95 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product GALE 蛋白
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- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as a 0.2 μm filtered solution of 50 mM TrisHCl, 150 mM NaCl, 2 mM DTT, 1 mM EDTA, pH 8.0.
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- GALE (UDP-Galactose-4-Epimerase (GALE))
- 别名
- GALE (GALE 产品)
- 背景
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Background: The enzyme UDP-Glucose 4-Epimerase (GALE) is a homodimeric epimerase found in bacterial, plant and mammalian cells. UDP-Glucose 4-Epimerase performs the final step in the Leloir pathway of Galactose metabolism, it catalyzes two distinct but analogous reactions: the epimerization of UDP-Gglucose to UDP-Galactose and the epimerization of UDP-N-Acetylglucosamine to UDP-N-Acetylgalactosamine. The bifunctional nature of the enzyme has the important metabolic consequence that mutant cells (or individuals) are dependent not only on exogenous galactose, but also on exogenous N-acetylgalactosamine as a necessary precursor for the synthesis of glycoproteins and glycolipids.
Synonym: UDP-Glucose 4-Epimerase, Galactowaldenase, UDP-Galactose 4-Epimerase, GALE
- 分子量
- 40.4 kDa
- UniProt
- Q14376
- 途径
- Response to Water Deprivation, Cellular Glucan Metabolic Process
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