UCHL3 Protein (His tag)
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- 抗原 See all UCHL3 (Uchl3) 蛋白
- UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 标记
- This UCHL3 protein is labelled with His tag.
- 原理
- Recombinant Human UCHL3/UCH-L3 Protein (His Tag)(Active)
- 序列
- Glu 2-Ala 230
- 产品特性
- A DNA sequence encoding the human UCHL3 (NP_005993.1) (Glu 2-Ala 230) was expressed, with a polyhistide tag at the N-terminus.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- Biological Activity Comment
- Measured by the hydrolysis of UbiquitinAMC. The specific activity is >14,000 pmoles/min/μg.
- Top Product
- Discover our top product Uchl3 蛋白
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 20 % glycerol, 1 mM DTT, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- UCHL3 (Uchl3) (Ubiquitin Carboxyl-terminal Esterase L3 (Ubiquitin Thiolesterase) (Uchl3))
- 别名
- UCHL3/UCH-L3 (Uchl3 产品)
- 别名
- UCH-L3 Protein, UCH-6 Protein, RGD1561196 Protein, im:6908825 Protein, zgc:109963 Protein, uchl4 Protein, ubiquitin C-terminal hydrolase L3 Protein, ubiquitin carboxyl-terminal esterase L3 (ubiquitin thiolesterase) Protein, ubiquitin C-terminal hydrolase L3 L homeolog Protein, UCHL3 Protein, Uchl3 Protein, uchl3 Protein, uchl3.L Protein
- 背景
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Background: Ubiquitin carboxyl-terminal hydrolase isozyme L3, also known as UCH-L3, Ubiquitin thioesterase L3 and UCHL3, is a ubiquitin-protein hydrolase which belongs to the peptidase C12 family. It is involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of either ubiquitin or NEDD8. UCHL3 is highly expressed in heart, skeletal muscle, and testis. UCHL1 and UCHL3 are two of the deubiquitinating enzymes expressed in the brain. These phenotypes indicate the importance of UCHL1 and UCHL3 in the regulation of the central nervous system. UCHL3 functions as a de-ubiquitinating enzyme where lack of its hydrolase activity may result in the prominent accumulation of ubiquitinated proteins and subsequent induction of stress responses in skeletal muscle. UCHL3 has also been identified as a tumor-specific antigen in colon cancer.
Synonym: Ubiquitin Carboxyl-Terminal Hydrolase Isozyme L3; UCH-L3; Ubiquitin Thioesterase L3; UCHL3
- 分子量
- 27 kDa
- NCBI登录号
- NP_005993
- 途径
- Feeding Behaviour, Positive Regulation of fat Cell Differentiation
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