PTPN12 蛋白
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- 抗原 See all PTPN12 蛋白
- PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 原理
- Recombinant Human PTPN12 Protein (Active)
- 序列
- Met 1-Gln355
- 产品特性
- A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.
- 纯度
- > 85 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to dephosphorylate a tyrosine residue in a peptide containing the EGFR Y992 phosphorylation site .The specific activity is >20 mol/min/mg
- Top Product
- Discover our top product PTPN12 蛋白
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Protein Tyrosine Phosphatase G1 (PTPN12) (AA 2-300) protein (His tag)
PTPN12 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Protein Tyrosine Phosphatase G1 (PTPN12) (AA 1-780) protein (GST tag)PTPN12 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Protein Tyrosine Phosphatase G1 (PTPN12) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PTPN12 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))
- 别名
- PTPN12 (PTPN12 产品)
- 背景
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Background: PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobilit.
Synonym: PTP-PEST,PTPG1
- 分子量
- 41.8 kDa
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