Pleiotrophin Protein (PTN)
-
- 抗原 See all Pleiotrophin (PTN) 蛋白
- Pleiotrophin (PTN)
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- Baculovirus infected Insect Cells
- 原理
- Recombinant Human Pleiotrophin/PTN/HB-GAM Protein (Active)
- 序列
- Met 1-Asp168
- 产品特性
- A DNA sequence encoding the mature form of human PTN (NP_002816.1) (Met1-Asp168) was expressed.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA. Immobilized human PTN at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc, The EC50 of rat SDC1-Fc is 0.35-0.81 μg/ml.
- Top Product
- Discover our top product PTN 蛋白
-
Pleiotrophin (PTN) (AA 33-168) protein (Fc Tag)
PTN 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Pleiotrophin (PTN) protein (His tag)PTN 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by reducing SDS-PAGE.
Pleiotrophin (PTN) (AA 45-154) protein (GST tag)PTN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Pleiotrophin (PTN) (AA 1-168) protein (GST tag)PTN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Pleiotrophin (PTN) (AA 33-168) protein (Fc Tag)PTN 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 90 % as determined by SDS-PAGE and Coomassie blue staining.
Pleiotrophin (PTN) (Active) proteinPTN 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 95 % as determined by SDS-PAGE Active
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 1M NaCl, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- Pleiotrophin (PTN)
- 别名
- Pleiotrophin/PTN/HB-GAM (PTN 产品)
- 别名
- zHBNF Protein, zgc:111787 Protein, harp Protein, hbnf Protein, X-PTF Protein, hbgf8 Protein, negf1 Protein, ptn Protein, PTN Protein, HARP Protein, HBGF8 Protein, HBNF Protein, NEGF1 Protein, PTN-A Protein, HB-GAM Protein, HBBM Protein, HBGF-8 Protein, OSF-1 Protein, HBBN Protein, OSF Protein, Osf-1 Protein, Osf1 Protein, Hbnf Protein, pleiotrophin Protein, pleiotrophin L homeolog Protein, ptn Protein, PTN Protein, ptn.L Protein, Ptn Protein
- 背景
-
Background: HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.
Synonym: HARP,HBGF8,HBNF,NEGF1
- 分子量
- 15.3 kDa
- NCBI登录号
- NP_002816
- 途径
- RTK signaling
-
