nectin-3 Protein (His tag)
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- 抗原 See all nectin-3 (NECTIN3) 蛋白
- nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This nectin-3 protein is labelled with His tag.
- 原理
- Recombinant Human Nectin-3/PVRL3 Protein (His Tag)(Active)
- 序列
- Met 1-Asp 400
- 产品特性
- A DNA sequence encoding the extracellular domain of human PVRL3 (NP_056295.1) (Met 1-Asp 400) was expressed, with a polyhistidine tag at the C-terminus.
- 纯度
- > 98 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its binding ability in a functional ELISA.Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml.2. Immobilized recombinant human PVRL3 at 1 μg/ml (100 μl/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5 ng/ml.
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- Discover our top product NECTIN3 蛋白
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Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) protein (His-Avi Tag)
NECTIN3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) protein (His-Avi Tag,Biotin)NECTIN3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) (Active) protein (Fc Tag)NECTIN3 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) (Active) protein (Fc Tag,AVI tag,Biotin)NECTIN3 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Nectin cell adhesion molecule 3 (NECTIN3) (AA 1-366) protein (GST tag)NECTIN3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nectin cell adhesion molecule 3 (NECTIN3) (AA 59-166) protein (GST tag)NECTIN3 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Nectin cell adhesion molecule 3 (NECTIN3) (AA 58-400) protein (His tag)NECTIN3 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- nectin-3 (NECTIN3) (Nectin cell adhesion molecule 3 (NECTIN3))
- 别名
- Nectin-3/PVRL3 (NECTIN3 产品)
- 别名
- PVRL3 Protein, CD113 Protein, CDW113 Protein, NECTIN-3 Protein, PPR3 Protein, PRR3 Protein, PVRR3 Protein, 2610301B19Rik Protein, 3000002N23Rik Protein, 4921513D19Rik Protein, AA407785 Protein, AU016832 Protein, AW538082 Protein, nectin cell adhesion molecule 3 Protein, poliovirus receptor-related 3 Protein, Nectin3 Protein, NECTIN3 Protein, PVRL3 Protein, nectin3 Protein
- 背景
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Background: Poliovirus receptor-related 3 (PVRL3), also known as Nectin-3 and CD113, is a member of the nectin family. PVRL3/Nectin-3 is an 83 kDa, type I transmembrane glycoprotein. Its precursor is 549 amino acids (aa) in length and contains an extended signal sequence of 57 aa, an extracellular domain (ECD) of 347 aa, a transmembrane segment of 21 aa, and a cytoplasmic region of 124 aa. Nectin-3 has three splicing variants, nectin-3alpha (biggest), -3beta (middle), and -3gamma (smallest). It is predominantly expressed in testis and placenta as well as in various cell lines, including epithelial cell lines. PVRL3/Nectin-3 plays a role in cell-cell adhesion through heterophilic trans-interactions with nectin-like proteins or nectins, such as trans-interaction with PVRL2/Nectin-2 at Sertoli-spermatid junctions. PVRL3/Nectin-3 is thus involved in the formation of cell-cell junctions, including adherens junctions and synapses. It has been shown to induce endocytosis-mediated down-regulation of PVR from the cell surface, resulting in reduction of cell movement and proliferation.
Synonym: Poliovirus Receptor-Related Protein 3, CDw113, Nectin-3, CD113, PVRL3, PRR3,PVRR3
- 分子量
- 39.3 kDa
- NCBI登录号
- NP_056295
- 途径
- Cell-Cell Junction Organization
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