ITCH 蛋白
-
- 抗原 See all ITCH 蛋白
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- 蛋白类型
- Recombinant
-
宿主
- 人
-
资源
- 大肠杆菌(E. Coli)
- 原理
- Recombinant Human ITCH/AIP4 Protein (aa 526-903)
- 序列
- Arg 526-Glu 903
- 产品特性
- A DNA sequence encoding the human ITCH (NP_113671.3) N-terminal segment (Arg 526-Glu 903) was expressed and purified, with two additional amino acids (Gly & Pro) at the N-terminus.
- 纯度
- > 98 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product ITCH 蛋白
-
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 200 mM NaCl, 10 % glycerol, pH 8.0
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- ITCH (Itchy E3 Ubiquitin Protein Ligase Homolog (ITCH))
- 别名
- ITCH/AIP4 (ITCH 产品)
- 别名
- AIF4 Protein, AIP4 Protein, NAPP1 Protein, dJ468O1.1 Protein, 6720481N21Rik Protein, 8030492O04Rik Protein, A130065M08 Protein, C230047C07Rik Protein, itchy E3 ubiquitin protein ligase Protein, itchy, E3 ubiquitin protein ligase Protein, ITCH Protein, Itch Protein
- 背景
-
Background: E3 ubiquitin-protein ligase Itchy homolog, also known as Atrophin-1-interacting protein 4, NFE2-associated polypeptide 1, NAPP1 and ITCH, is a cell membrane protein which contains one C2 domain, one HECT (E6AP-type E3 ubiquitin-protein ligase) domain and contains four WW domains. ITCH acts as an E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It catalyzes 'Lys-29'-, 'Lys-48'- and 'Lys-63'-linked ubiquitin conjugation. ITCH is involved in the control of inflammatory signaling pathways. It is an essential component of a ubiquitin-editing protein complex, comprising also TNFAIP3, TAX1BP1 and RNF11, that ensures the transient nature of inflammatory signaling pathways. ITCH promotes the association of the complex after TNF stimulation. Once the complex is formed, TNFAIP3 deubiquitinates 'Lys-63' polyubiquitin chains on RIPK1 and catalyzes the formation of 'Lys-48'-polyubiquitin chains. This leads to RIPK1 proteosomal degradation and consequently termination of the TNF- or LPS-mediated activation of NFKB1. Defects in ITCH are the cause of syndromic multisystem autoimmune disease (SMAD) which is characterized by organomegaly, failure to thrive, developmental delay, dysmorphic features and autoimmune inflammatory cell infiltration of the lungs, liver and gut.
Synonym: ADMFD;AIF4;AIP4;dJ468O1.1;NAPP1
- 分子量
- 40 kDa
- NCBI登录号
- NP_113671
- 途径
- Activation of Innate immune Response, CXCR4-mediated Signaling Events
-