GFRA1 Protein (AA 1-424) (His tag)
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- 抗原 See all GFRA1 蛋白
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
- 产品特性
- AA 1-424
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This GFRA1 protein is labelled with His tag.
- 原理
- Recombinant Human GFRA1/GDNFRA Protein (aa 1-424, His Tag)(Active)
- 序列
- Met 1-Ser 424
- 产品特性
- A DNA sequence encoding the human GFRα1 without the propeptide (NP_665736.1) (Met 1-Ser 424) was fused with the a polyhistidine tag at the C-terminus.
- 纯度
- > 98 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- 1. Measured in a cell proliferation assay using SH-SY5Y human neuroblastoma cells. The ED50 for this effect is typically 0.2-1 μg/mL in the presence of 40 ng/mL Recombinant Human GDNF.2. Measured by its ability to bind human GDNF (native) in functional ELISA.
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- Discover our top product GFRA1 蛋白
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GDNF Family Receptor alpha 1 (GFRA1) (AA 25-424) protein (His tag)
GFRA1 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
GDNF Family Receptor alpha 1 (GFRA1) (AA 267-360) protein (GST tag)GFRA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
GDNF Family Receptor alpha 1 (GFRA1) (AA 1-460) protein (GST tag)GFRA1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (His tag)GFRA1 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 98 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (His tag)GFRA1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 97 % as determined by SDS-PAGE Active
GDNF Family Receptor alpha 1 (GFRA1) (Active) protein (Fc Tag)GFRA1 宿主: 大鼠 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE Active
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- GFRA1 (GDNF Family Receptor alpha 1 (GFRA1))
- 别名
- GFRA1/GDNFRA (GFRA1 产品)
- 别名
- gdnfr Protein, ret1l Protein, retl1 Protein, trnr1 Protein, gdnfra Protein, MGC122616 Protein, gfr-alpha-1 Protein, GDNFR Protein, GDNFRA Protein, GFR-ALPHA-1 Protein, RET1L Protein, RETL1 Protein, TRNR1 Protein, GDNFRALPHA Protein, GFRA1 Protein, AU042498 Protein, gfralpha1a Protein, zgc:110129 Protein, GDNF family receptor alpha 1 Protein, glial cell line derived neurotrophic factor family receptor alpha 1 Protein, gdnf family receptor alpha 1a Protein, gfra1 Protein, GFRA1 Protein, Gfra1 Protein, gfra1a Protein
- 背景
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Background: Glial cell line derived neurotrophic factor (GDNF) Family Receptor Alpha 1 (GFRA1) is a member of the GDNF receptor family. It is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. GFRA1 is a potent survival factor for central and peripheral neurons, and is essential for the development of kidneys and the enteric nervous system. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are its binding ligand which are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. GDNF promotes the formation of a physical complex between GFRA/GDNFRa and the orphan tyrosin kinase receptor Ret, thereby inducing its tyrosine phosphorylation. The RET is a receptor tyrosine kinase representing the signal-transducing molecule of a multisubunit surface receptor complex for the GDNF, in which GFRA / GDNFRa acts as the ligand-binding component. GDNF, a distantly related member of the transforming growth factor-β (TGF-â) superfamily, and its receptor components: GFRA1, Ret and neural cell adhesion molecule (NCAM) have been recently reported to be expressed in the testis and to be involved in the proliferation regulation of immature Sertoli cells.
Synonym: GDNF Family Receptor Alpha-1; GDNF Receptor Alpha-1; GDNFR-Alpha-1; GFR-Alpha-1; RET Ligand 1; TGF-Beta-Related Neurotrophic Factor Receptor 1; GFRA1; GDNFRA; RETL1; TRNR1
- 分子量
- 46 kDa
- NCBI登录号
- NP_665736
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