Chitotriosidase 1 Protein (His tag)
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- 抗原 See all Chitotriosidase 1 (CHIT1) 蛋白
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This Chitotriosidase 1 protein is labelled with His tag.
- 原理
- Recombinant Human Chitotriosidase/CHIT1 Protein (His Tag)
- 序列
- Met 1-Asn 466
- 产品特性
- A DNA sequence encoding the human CHIT1 (NP_003456.1) (Met 1-Asn 466) precursor was fused with a polyhistidine tag at the C-terminus.
- 纯度
- > 94 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product CHIT1 蛋白
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Chitotriosidase (CHIT1) (AA 1-466) protein (GST tag)
CHIT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chitotriosidase (CHIT1) (AA 60-169) protein (GST tag)CHIT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Chitotriosidase (CHIT1) protein (Myc-DYKDDDDK Tag)CHIT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 12.5 mM Tris, 75 mM NaCl, 50 % glycerol, pH 7.5
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- Chitotriosidase 1 (CHIT1) (Chitotriosidase (CHIT1))
- 别名
- Chitotriosidase/CHIT1 (CHIT1 产品)
- 别名
- CHI3 Protein, CHIT Protein, CHITD Protein, CHIT1 Protein, chio Protein, 2300002L19Rik Protein, chitinase 1 Protein, chitinase 1 (chitotriosidase) Protein, CHIT1 Protein, chit1 Protein, Chit1 Protein
- 背景
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Background: Chitotriosidase, also known as Chitinase-1 and CHIT1, is a member of the glycosyl hydrolase 18 family and Chitinase class II subfamily. It is a member of the mammalian chitinase family, structurally homologous to chitinases from other species, is synthesized and secreted by specifically activated macrophages. Chitotriosidase is a polymer of N-acetylglucosamine. Serum and plasma chitotriosidase activity is usually measured as the first step in diagnosis of Gaucher disease. Monitoring chitotriosidase activity is widely used during treatment of this pathology by enzyme replacement therapy. Its elevated plasma level reflects gradual intralysosomal accumulation in Gaucher cells (lipid-loaded macrophages). Macrophages overloaded by the enzyme accumulated in lysosomal material (lipids) were shown to secrete chitotriosidase, its increased expression was noted in several lysosomal storage diseases and atherosclerosis. In addition to lipid storage disorders, where Chit activity has longer been used as a marker of disease activity and therapeutic response, elevation of plasma Chit may occur in hematological disorders with storage of erythrocyte membrane breakdown products as thalassemia and different systemic infectious diseases sustained by fungi and other pathogens. Recently, increased Chit activity was demonstrated in CNS from patients with different neurological disorders. Chitotriosidase is believed to play a role in mechanisms of immunity and protection against chitin-containing pathogens.
Synonym: Chitotriosidase-1, Chitinase-1, CHIT1,CHI3,CHIT,CHITD
- 分子量
- 50.8 kDa
- NCBI登录号
- NP_003456
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