CHEK1 Protein (GST tag)
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- 抗原 See all CHEK1 蛋白
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- Baculovirus infected Insect Cells
- 标记
- This CHEK1 protein is labelled with GST tag.
- 原理
- Recombinant Human CHK1/CHEK1 Protein (GST Tag)(Active)
- 序列
- Met 1-Thr476
- 产品特性
- A DNA sequence encoding the human CHK1 (AAM78553.1) (Met1-Thr476) was fused with the GST tag at the N-terminus.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 32 nmol/min/mg using CHKtide peptide (KKKVSRSGLYRSPSMPENLNRPR) as substrate.
- Top Product
- Discover our top product CHEK1 蛋白
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Checkpoint Kinase 1 (CHEK1) (AA 361-476) protein (GST tag)
CHEK1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Checkpoint Kinase 1 (CHEK1) protein (GST tag,His tag)CHEK1 宿主: 小鼠 宿主: Baculovirus infected Insect Cells Recombinant > 90 % as determined by SDS-PAGE
Checkpoint Kinase 1 (CHEK1) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)CHEK1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Frozen, Liquid
- 缓冲液
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 2 mM GSH
- 储存条件
- -20 °C
- 储存方法
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- 抗原
- CHEK1 (Checkpoint Kinase 1 (CHEK1))
- 别名
- CHK1/CHEK1 (CHEK1 产品)
- 别名
- An08g10320 Protein, AO090003000441 Protein, CHK1 Protein, chk1 Protein, C85740 Protein, Chk1 Protein, rad27 Protein, id:ibd2720 Protein, zgc:56093 Protein, CHEK1 Protein, checkpoint kinase 1 Protein, serine/threonine-protein kinase chk1 Protein, serine/threonine-protein kinase Chk1 Protein, CAMK/CAMKL/CHK1 protein kinase Chk1 Protein, checkpoint kinase 1 S homeolog Protein, CHEK1 Protein, ANI_1_2436074 Protein, AOR_1_768154 Protein, PTRG_04183 Protein, SJAG_01680 Protein, PAAG_04978 Protein, MCYG_03290 Protein, VDBG_03742 Protein, chek1.S Protein, Chek1 Protein, chek1 Protein
- 背景
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Background: CHK1 / CHEK1 contains 1 protein kinase domain and belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family, NIM1 subfamily. It is a member of checkpoint kinases (Chks). Chks Checkpoint kinases (Chks) are serine/threonine kinases that are involved in the control of the cell cycle. There are two subtypes of chks that have so far been identified, CHK1 / CHEK1 and Chk2. They are essential components to delay cell cycle progression in normal and damaged cells and can act at all three cell cycle checkpoints. Chks are activated by phosphorylation. ATR kinase phosphorylates CHK1 / CHEK1 in response to single strand DNA breaks and ATM kinase phosphorylates Chk2 in response to double strand breaks. Chks phosphorylate Cdc25 phosphatase at Ser216, which leads to Cdc25 sequestration in the cytoplasm. Chks have a role in the physiological stress of hypoxia/reoxygenation. CHK1 / CHEK1 is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. CHK1 / CHEK1 may also negatively regulate cell cycle progression during unperturbed cell cycles.Immune Checkpoint Immunotherapy Cancer Immunotherapy Targeted Therapy
Synonym: CHK1
- 分子量
- 80.7kDa
- 途径
- p53 Pathway, Apoptosis, Cell Division Cycle, DNA Damage Repair
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