CA12 Protein (His tag)
-
- 抗原 See all CA12 蛋白
- CA12 (Carbonic Anhydrase 12 (CA12))
- 蛋白类型
- Recombinant
- 生物活性
- Active
-
宿主
- 人
-
资源
- HEK-293 Cells
- 标记
- This CA12 protein is labelled with His tag.
- 原理
- Recombinant Human Carbonic Anhydrase XII/CA12 Protein (His Tag)(Active)
- 序列
- Met 1-Gln 291
- 产品特性
- A DNA sequence encoding the extracellular domain of human CA12 (NP_001209.1) (Met 1-Gln 291) was expressed with a C-terminal polyhistidine tag.
- 纯度
- > 97 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its esterase activity. The specific activity is >40 pmoles/min/μg, as measured with 1 mM 4-Nitrophenyl acetate and 2.5 μg enzyme at 400 nm in 100 μL of 12.5 mM Tris, 75 mM NaCl, pH 7.5.
- Top Product
- Discover our top product CA12 蛋白
-
Carbonic Anhydrase 12 (CA12) (AA 25-301) protein (His tag)
CA12 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase 12 (CA12) (AA 25-299) protein (His tag)CA12 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Carbonic Anhydrase 12 (CA12) (AA 1-354) protein (GST tag)CA12 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase 12 (CA12) (AA 25-124) protein (GST tag)CA12 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Carbonic Anhydrase 12 (CA12) protein (His tag)CA12 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE
Carbonic Anhydrase 12 (CA12) (Active) protein (His tag)CA12 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Carbonic Anhydrase 12 (CA12) (AA 25-301) protein (Fc Tag)CA12 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
-
- 限制
- 仅限研究用
-
- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- 抗原
- CA12 (Carbonic Anhydrase 12 (CA12))
- 别名
- Carbonic Anhydrase XII/CA12 (CA12 产品)
- 别名
- CAXII Protein, HsT18816 Protein, CAR12 Protein, 2310047E01Rik Protein, AI314958 Protein, Ca12 Protein, ca12 Protein, carbonic anhydrase 12 Protein, carbonic anhydrase XII L homeolog Protein, CA12 Protein, Car12 Protein, ca12.L Protein
- 背景
-
Background: Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes first discovered in 1933 that catalyze the reversible hydration of carbon dioxide. CAs participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. CA12, also known as Car12 and carbonic anhydrase XII, is a type I membrane enzyme of an N-terminal extracellular catalytic domain, a membrane-spanning α-helix, and a small intracellular C-terminal domain. It is highly expressed in colon, kidney, prostate, intestine and activated lymphocytes and moderately expressed in pancreas, ovary, and testis. Overexpression of the CA12 is observed in certain human cancers and is used as a tumor marker. rmCA12 corresponds to the extracellular domain and has both carbonic anhydrase activity and esterase activity.
Synonym: CA12;CAXII;FLJ20151;HsT18816
- 分子量
- 31.6 kDa
- NCBI登录号
- NP_001209
-
