BACE1 Protein (Fc Tag)
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- 抗原 See all BACE1 蛋白
- BACE1 (Beta-secretase 1 (BACE1))
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This BACE1 protein is labelled with Fc Tag.
- 原理
- Recombinant Human BACE1/ASP2 Protein (Fc Tag)(Active)
- 序列
- Met 1-Thr 457
- 产品特性
- A DNA sequence encoding the extracellular domain (Met 1-Thr 457) of human BACE1 (NP_036236.1) was expressed as a secreted chimeric protein with the C-terminal fused Fc region of human IgG1.
- 纯度
- > 90 % as determined by reducing SDS-PAGE.
- 内毒素水平
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a fluorogenic peptide substrate, Mca-SEVNLDAEFRK(Dpn)RR-NH2, (R&D Systems, Catalog # ES004). The specific activity is >0.5 pmoles/min/μg.
- Top Product
- Discover our top product BACE1 蛋白
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile PBS, pH 7.4
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- BACE1 (Beta-secretase 1 (BACE1))
- 别名
- BACE1 (BACE1 产品)
- 别名
- ASP2 Protein, BACE Protein, HSPC104 Protein, C76936 Protein, Bace Protein, MGC145931 Protein, BACE1 Protein, zgc:77409 Protein, beta-secretase 1 Protein, beta-site APP cleaving enzyme 1 Protein, beta-site APP-cleaving enzyme 1 Protein, BACE1 Protein, Bace1 Protein, bace1 Protein
- 背景
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Background: Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca(2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.
Synonym: ASP2,BACE,HSPC104
- 分子量
- 75 kDa
- NCBI登录号
- NP_036236
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