AGO2 Protein (His tag)
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- 抗原 See all AGO2 蛋白
- AGO2 (Argonaute 2 (AGO2))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- Baculovirus infected Insect Cells
- 标记
- This AGO2 protein is labelled with His tag.
- 原理
- Recombinant Mouse AGO2/Argonaute 2/EIF2C2 Protein (His Tag)
- 序列
- Met 1-Ala 860
- 产品特性
- A DNA sequence encoding the mouse AGO2 (Q8CJG0) (Met 1-Ala 860) was fused with a polyhistidine tag at the N-terminus.
- 纯度
- > 88 % as determined by SDS-PAGE
- 内毒素水平
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Top Product
- Discover our top product AGO2 蛋白
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Argonaute 2 (AGO2) (AA 381-480) protein (GST tag)
AGO2 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
Argonaute 2 (AGO2) protein (DYKDDDDK Tag)AGO2 宿主: 人 宿主: Baculovirus Recombinant RNAi, ScA
Argonaute 2 (AGO2) (AA 517-818) protein (His tag)AGO2 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Argonaute 2 (AGO2) (AA 517-818) protein (His tag)AGO2 宿主: 人 宿主: 酵母菌 Recombinant > 90 % SDS
Argonaute 2 (AGO2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)AGO2 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
- Please refer to the printed manual for detailed information.
- 缓冲液
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol
- 储存条件
- 4 °C,-20 °C,-80 °C
- 储存方法
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- 抗原
- AGO2 (Argonaute 2 (AGO2))
- 别名
- AGO2/Argonaute 2/EIF2C2 (AGO2 产品)
- 别名
- EIF2C2 Protein, T19E23.7 Protein, T19E23_7 Protein, argonaute 2 Protein, Q10 Protein, Eif2c2 Protein, Argonaute2 Protein, eif2c1 Protein, eif2c2 Protein, 1110029L17Rik Protein, 2310051F07Rik Protein, AI225898 Protein, AL022874 Protein, AW546247 Protein, ENSMUSG00000072493 Protein, Gerp95 Protein, Gm10365 Protein, mKIAA4215 Protein, AGO2 Protein, AG02 Protein, AGO 2 Protein, Ago-2 Protein, Ago2 Protein, CG13452 Protein, CG7439 Protein, Dm Ago2 Protein, Dmel\\CG7439 Protein, ago Protein, ago-2 Protein, ago2 Protein, dAGO2 Protein, dAgo2 Protein, eIF2C2 Protein, argonaute 2, RISC catalytic component Protein, Argonaute family protein Protein, argonaute 2, RISC catalytic component L homeolog Protein, argonaute RISC catalytic subunit 2 Protein, argonaute RISC catalytic component 2 Protein, Argonaute 2 Protein, AGO2 Protein, Ago2 Protein, ago2.L Protein, ago2 Protein
- 背景
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Background: Argonaute 2 (AGO2), also known as Eukaryotic translation initiation factor 2C2 (EIF2C2), belongs to the Argonaute family, AGO subfamily, which is a component of the RNA-induced silencing complex (RISC) and mediates small interfering RNA (siRNA)-directed mRNA cleavage and microRNA translational suppression. AGO2 protein is the catalytic engine of mammalian RNAi. It contains a PIWI domain that is structurally related to RNases H and possibly shares with them a two-metal-ion catalysis mechanism. Human AGO2 was unable to cleave preformed RNA duplexes and exhibited weaker binding affinity for RNA duplexes compared with the single strand RNA. The enzyme exhibited greater RNase H activity in the presence of Mn2+ compared with Mg2+. Human AGO2 exhibited weaker binding affinities and reduced cleavage activities for antisense RNAs with either a 5'-terminal hydroxyl or abasic nucleotide. In mouse hematopoiesis, AGO2 controls early development of lymphoid and erythroid cells. AGO2 is a highly specialized member of the Argonaute family with an essential nonredundant Slicer-independent function within the mammalian miRNA pathway. AGO2 regulates dFMR1 expression, and the relationship between dFMR1 and AGO2 was defined by their physical interaction and co-regulation of downstream targets. AGO2 and dFMR1 are also connected through a regulatory relationship. AGO2 is a regulator of dFMR1 expression and have clarified an important developmental role for AGO2 in the nervous system and germ line that requires dFMR1 function. In addition, AGO2 is regulated at both the transcriptional and posttranslational level, and also implicate AGO2 and enhanced micro-RNA activity in the tumorigenic progression of breast cancer cell lines.
Synonym: 1110029L17Rik,2310051F07Rik,AI225898,AL022874,AW546247,Eif2c2,ENSMUSG00000072493,Gerp95,Gm10365,mKIAA4215
- 分子量
- 99 kDa
- UniProt
- Q8CJG0
- 途径
- Fc-epsilon Receptor Signaling Pathway, Regulatory RNA Pathways, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Ribonucleoprotein Complex Subunit Organization
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