ApoE3 Protein (Arg154Ser-Mutant) (His-Avi Tag,Biotin)
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- 抗原 See all ApoE3 products
- ApoE3 (Apolipoprotein E3 (ApoE3))
- 蛋白类型
- Recombinant
- 产品特性
- Arg154Ser-Mutant
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ApoE3 protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human Apolipoprotein E / APOE3 (R154S) Protein, His,Avitag™ (MALS verified)
- 序列
- Lys 19 - His 317
- 产品特性
- Biotinylated Human APOE3 (R154S), His,Avitag is expressed from human 293 cells (HEK293). It contains AA Lys 19 - His 317 (Accession # P02649-1 (R154S).
- 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
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Apolipoprotein E3 (ApoE3) (AA 19-317) protein (His tag)
ApoE3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Apolipoprotein E3 (ApoE3) (AA 19-317) protein (His tag,Biotin)ApoE3 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
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- 应用备注
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 37.7 kDa. The protein migrates as 39-44 kDa under reducing (R) condition due to glycosylation.
- 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, 5 mM CHAPS, 2 mM DTT, pH 7.4
- 储存条件
- -20 °C
- 储存方法
- For long term storage, the product should be stored at lyophilized state at -20°C or lower.
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- 抗原
- ApoE3 (Apolipoprotein E3 (ApoE3))
- 别名
- Apolipoprotein E / APOE3 (ApoE3 产品)
- 别名
- AD2 Protein, LDLCQ5 Protein, LPG Protein, apolipoprotein E Protein, APOE Protein
- 背景
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Synonyms: Apolipoprotein E,APOE,Apo-E,
Description: APOE is an apolipoprotein, a protein associating with lipid particles, that mainly functions in lipoprotein-mediated lipid transport between organs via the plasma and interstitial fluids. APOE is a core component of plasma lipoproteins and is involved in their production, conversion and clearance. Apoliproteins are amphipathic molecules that interact both with lipids of the lipoprotein particle core and the aqueous environment of the plasma. As such, APOE associates with chylomicrons, chylomicron remnants, very low density lipoproteins (VLDL) and intermediate density lipoproteins (IDL) but shows a preferential binding to high-density lipoproteins (HDL). It also binds a wide range of cellular receptors including the LDL receptor/LDLR, the LDL receptor-related proteins LRP1, LRP2 and LRP8 and the very low-density lipoprotein receptor/VLDLR that mediate the cellular uptake of the APOE-containing lipoprotein particles. - 分子量
- 37.7 kDa
- 途径
- Regulation of Cell Size, Lipid Metabolism
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