SIGLEC7 Protein (His-Avi Tag,Biotin)
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- 抗原 See all SIGLEC7 蛋白
- SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This SIGLEC7 protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human Siglec-7 / CD328 Protein, His,Avitag™ (MALS & SPR verified)
- 序列
- Gln 19 - Leu 353
- 产品特性
- Biotinylated Human Siglec-7, His,Avitag (SG7-H82E7) is expressed from human 293 cells (HEK293). It contains AA Gln 19 - Leu 353 (Accession # Q9Y286-1).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
- Top Product
- Discover our top product SIGLEC7 蛋白
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Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 1-467) protein (GST tag)
SIGLEC7 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 377-466) protein (GST tag)SIGLEC7 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 19-353) protein (His tag)SIGLEC7 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 85 % as determined by SDS-PAGE and Coomassie blue staining.
Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 19-353) (Active) protein (His tag)SIGLEC7 宿主: 人 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7) (AA 19-353) protein (hIgG-His-tag)SIGLEC7 宿主: 人 宿主: Baculovirus infected Insect Cells Recombinant > 90 % by SDS - PAGE SDS
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- 应用备注
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 40.5 kDa. The protein migrates as 55-65 kDa under reducing (R) condition due to glycosylation.
- 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- 25 mM MES, 150 mM NaCl, pH 5.5
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- SIGLEC7 (Sialic Acid Binding Ig-Like Lectin 7 (SIGLEC7))
- 别名
- Siglec-7 / CD328 (SIGLEC7 产品)
- 别名
- SIGLEC7 Protein, AIRM1 Protein, CD328 Protein, CDw328 Protein, D-siglec Protein, QA79 Protein, SIGLEC-7 Protein, SIGLEC19P Protein, SIGLECP2 Protein, p75 Protein, p75/AIRM1 Protein, sialic acid-binding Ig-like lectin 7 Protein, sialic acid binding Ig like lectin 7 Protein, LOC468976 Protein, SIGLEC7 Protein
- 背景
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Synonyms: CDw328,D-siglec,A79 membrane protein,p75,Adhesion inhibitory receptor molecule 1, AIRM-1,
Description: Siglec-7 is a member of the human CD33-related Siglec receptor. The extracellular region of Siglec-7 is characterized by an N-terminal V-set Ig domain that can bind sialic acid and two C2-set Ig domains. The cytoplasmic tail of Siglec-7 has one immune-receptor tyrosine-based inhibitory motif (ITIM) and one ITIM-like motif. Siglec-7 is considered as a sialic acid-dependent immunoreceptor with inhibitory potential and expressed predominantly on human NK cells, monocytes and a small subset of CD8+ T cells. - 分子量
- 40.5 kDa
- NCBI登录号
- NP_055200
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