ROBO1 Protein (His-Avi Tag,Biotin)
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- 抗原 See all ROBO1 蛋白
- ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- HEK-293 Cells
- 标记
- This ROBO1 protein is labelled with His-Avi Tag,Biotin.
- 原理
- Biotinylated Human ROBO1 Protein, His,Avitag™ (MALS verified)
- 序列
- GIn 26 - Pro 897
- 产品特性
- Biotinylated Human ROBO1, His,Avitag (RB1-H82E5) is expressed from human 293 cells (HEK293). It contains AA GIn 26 - Pro 897 (Accession # Q9Y6N7-1).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- 质量等级
- MALS verified
- Top Product
- Discover our top product ROBO1 蛋白
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- 应用备注
- This protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™). The protein has a calculated MW of 99.2 kDa. The protein migrates as kDa under reducing (R) condition due to glycosylation.
- 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -20 °C
- 储存方法
- -20°C
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- 抗原
- ROBO1 (Roundabout, Axon Guidance Receptor, Homolog 1 (ROBO1))
- 别名
- ROBO1 (ROBO1 产品)
- 别名
- DUTT1 Protein, SAX3 Protein, AW494633 Protein, AW742721 Protein, Gm310 Protein, ROBO1 Protein, dutt1 Protein, roundabout Protein, sax3 Protein, MGC122264 Protein, roundabout-1 Protein, roundabout guidance receptor 1 Protein, roundabout, axon guidance receptor, homolog 1 (Drosophila) Protein, roundabout guidance receptor 1 L homeolog Protein, ROBO1 Protein, Robo1 Protein, robo1 Protein, robo1.L Protein
- 背景
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Synonyms: ROBO1/DUTT1,
Description: ROBO1 is a member of the ROBO immunoglobulin superfamily of proteins, and it plays a crucial role in cell motility and migration during embryogenesis and organogenesis. In addition, evidence showed that ROBO1 might drive migration and invasion in malignant cells, such as glioma and breast cancer, which might play a role in cancer aggressiveness. In contrast, some studies suggested that ROBO1 pathways play a key role in tumors by acting as a tumor suppressor, especially in cell invasion. - 分子量
- 99.2 kDa
- NCBI登录号
- NP_002932
- 途径
- Positive Regulation of Endopeptidase Activity
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