SARS-CoV-2 Spike S2 Protein (His tag,AVI tag,Biotin)
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- 抗原 See all SARS-CoV-2 Spike S2 蛋白
- SARS-CoV-2 Spike S2
- 蛋白类型
- Recombinant
- 生物活性
- Active
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宿主
- SARS Coronavirus-2 (SARS-CoV-2)
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资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Spike S2 protein is labelled with His tag,AVI tag,Biotin.
- 序列
- AA 686-1213
- 特异性
- Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
- 产品特性
- Biotinylated SARS-CoV-2 S2 protein, His,Avitag™ is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1).
- 纯度
- >95 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
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- Discover our top product SARS-CoV-2 Spike S2 蛋白
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- 说明
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Ready-to-use Avitag™ biotinylated protein:
The product is exclusively produced using the Avitag™ technology. Briefly, a unique 15 amino acid peptide, the Avi tag, is introduced into the recombinant protein during expression vector construction. The single lysine residue in the Avi tag is enzymatically biotinylated by the E. Coli biotin ligase BirA.
This single-point enzymatic labeling technique brings many advantages for commonly used binding assays. The biotinylation happens on the lysine residue of Avi tag, and therefore does NOT interfere with the target protein's natural binding activities. In addition, when immobilized on an avidin-coated surface, the protein orientation is uniform because the position of the Avi tag in the protein is precisely controlled. - 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 储存条件
- -20 °C
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- 抗原
- SARS-CoV-2 Spike S2
- 别名
- SARS-CoV-2 S2 protein (SARS-CoV-2 Spike S2 产品)
- 别名
- E2 Protein, Surface Glycoprotein Protein, S Protein
- 物质类
- Viral Protein
- 背景
- It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- 分子量
- 61.5 kDa
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