This SARS-CoV-2 Spike protein is labelled with His tag.
应用范围
ELISA
原理
SARS-CoV-2 S protein, His Tag, Super stable trimer (MALS & NS-EM verified)
序列
AA 16-1213
产品特性
SARS-CoV-2 S protein, His Tag, Super stable trimer is the ectodomain of SARS-CoV-2 S protein which contains AA Val 16 - Pro 1213 (Accession # QHD43416.1). The recombinant protein is expressed from human 293 cells (HEK293) with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (F817P, A892P, A899P, A942P, K986P, V987P) and alanine substitutions (R683A and R685A) are introduced to stabilize the trimeric prefusion state of SARS-CoV-2 S protein and abolish the furin cleavage site, respectively.
纯度
> 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE
应用备注
This product is specifically designed for antibody drug development.
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS
储存条件
-20 °C,-80 °C
储存方法
For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable after storage at: -20°C to -70°C for 12 months in lyophilized state 70°C for 3 months under sterile conditions after reconstitution.
有效期
12 months
Yokoyama, Ichiki: "Nano-size dependence in the adsorption by the SARS-CoV-2 spike protein over gold colloid." in: Colloids and surfaces. A, Physicochemical and engineering aspects, Vol. 615, pp. 126275, (2021) (PubMed).
E2 Protein, E2 glycoprotein precursor Protein, Surface Glycoprotein Protein, S Protein
物质类
Viral Protein
背景
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.