This SARS-CoV-2 Spike S1 protein is labelled with His tag.
应用范围
SDS-PAGE (SDS), ELISA
原理
SARS-CoV-2 (COVID-19) S protein RBD (W436R), His Tag
序列
AA 319-541
产品特性
SARS-CoV-2 S protein RBD (W436R), His Tag (SPD-S52H7) is expressed from human 293 cells (HEK293). It contains AA Arg 319 - Phe 541 (Accession # QHD43416.1 (W436R). Predicted N-terminus: Arg 319 This protein carries a polyhistidine tag at the C-terminus. The W436R mutation in the RBD of Spike protein shows a higher binding affinity to human ACE2.
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS, pH 7.4
注意事项
Please avoid repeated freeze-thaw cycles.
储存条件
4 °C,-20 °C,-80 °C
储存方法
For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable after storage at: 4-8°C for 12 months in lyophilized state, -70°C for 3 years under sterile conditions after reconstitution.
E2 Protein, Surface Glycoprotein Protein, S Protein
物质类
Viral Protein
背景
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.