This SARS-CoV-2 Spike S2 protein is labelled with His tag.
应用范围
SDS-PAGE (SDS)
原理
SARS-CoV-2 (COVID-19) S2 protein, His Tag
序列
AA 686-1213
产品特性
SARS-CoV-2 S2 protein, His Tag is expressed from human 293 cells (HEK293). It contains AA Ser 686 - Pro 1213 (Accession # QHD43416.1). Predicted N-terminus: Ser 686 This protein carries a polyhistidine tag at the C-terminus.
宿主: SARS Coronavirus-2 (SARS-CoV-2), SARS CoV-2 Omicron
宿主: HEK-293 Cells
Recombinant
>95 % as determined by SDS-PAGE.
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
缓冲液
PBS, pH 7.4
注意事项
Please avoid repeated freeze-thaw cycles.
储存条件
4 °C,-20 °C,-80 °C
储存方法
For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable after storage at: 4-8°C for 12 months in lyophilized state, -70°C for 3 years under sterile conditions after reconstitution.
Longworth, Dittmar: "An antigen microarray protocol for COVID-19 serological analysis." in: STAR protocols, Vol. 2, Issue 3, pp. 100815, (2021) (PubMed).
Tosif, Neeland, Sutton, Licciardi, Sarkar, Selva, Do, Donato, Quan Toh, Higgins, Van de Sandt, Lemke, Lee, Shoffner, Flanagan, Arnold, Mordant, Mulholland, Bines, Dohle, Pellicci, Curtis, McNab et al.: "Immune responses to SARS-CoV-2 in three children of parents with symptomatic COVID-19. ..." in: Nature communications, Vol. 11, Issue 1, pp. 5703, (2020) (PubMed).
E2 Protein, Surface Glycoprotein Protein, S Protein
物质类
Viral Protein
背景
It's been reported that SARS-CoV-2 can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.