This SARS-CoV-2 Spike S1 protein is labelled with Fc Tag.
应用范围
SDS-PAGE (SDS), ELISA
原理
SARS-CoV-2 (COVID-19) S1 protein, Fc Tag
序列
AA 16-685
产品特性
SARS-CoV-2 S1 protein, Fc Tag is expressed from human 293 cells (HEK293). It contains AA Val 16 - Arg 685 (Accession # QHD43416.1). Predicted N-terminus: Val 16 This protein carries a human IgG1 Fc tag at the C-terminus.
宿主: SARS Coronavirus-2 (SARS-CoV-2)
宿主: HEK-293 Cells
Recombinant
> 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
应用备注
Optimal working dilution should be determined by the investigator.
限制
仅限研究用
状态
Lyophilized
缓冲液
Tris, Glycine and NaCl, pH 7.5
注意事项
Please avoid repeated freeze-thaw cycles.
储存条件
4 °C,-20 °C,-80 °C
储存方法
For long term storage, the product should be stored at lyophilized state at -20°C or lower. This product is stable after storage at: 4-8°C for 12 months in lyophilized state, -70°C for 3 years under sterile conditions after reconstitution.
Dogan, Kozhaya, Placek, Gunter, Yigit, Hardy, Plassmeyer, Coatney, Lillard, Bukhari, Kleinberg, Hayes, Arditi, Klapper, Merin, Liang, Gupta, Alpan, Unutmaz: "SARS-CoV-2 specific antibody and neutralization assays reveal the wide range of the humoral immune response to virus." in: Communications biology, Vol. 4, Issue 1, pp. 129, (2021) (PubMed).
It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.