SARS-CoV-2 Spike S1 Protein (His tag)
(9 references)-
- 抗原 See all SARS-CoV-2 Spike S1 蛋白
- SARS-CoV-2 Spike S1
- 蛋白类型
- Recombinant
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宿主
- SARS Coronavirus-2 (SARS-CoV-2)
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资源
- HEK-293 Cells
- 标记
- This SARS-CoV-2 Spike S1 protein is labelled with His tag.
- 应用范围
- SDS-PAGE (SDS), Functional Studies (Func)
- 序列
- AA 16-685
- 产品特性
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This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 76.9 kDa. The protein migrates as 100-140 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
AA 16-685 - 纯度
- >90 % as determined by SDS-PAGE.
- 内毒素水平
- Less than 1.0 EU per μg by the LAL method.
- Biological Activity Comment
- Immobilized 2019-nCoV S1 protein, His Tag at 2 μg/mL (100 μL/well) can bind Human ACE2, Fc Tag with a linear range of 2-20 ng/mL (QC tested).
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SARS-CoV-2 Spike S1 (RBD) protein (His tag,MYC tag)
宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: Mammalian Cells Recombinant Greater than 90 % as determined by SDS-PAGE.
SARS-CoV-2 Spike S1 (B.1.1.529 - Omicron) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (RBD) protein (His tag,AVI tag,Biotin)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 90 % as determined by SDS-PAGE. > 90 % as determined by SEC-MALS.
SARS-CoV-2 Spike S1 (N501Y) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 protein (Fc-Avi Tag)宿主: SARS Coronavirus (SARS-CoV) 宿主: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE,> 95% as determined by HPLC
SARS-CoV-2 Spike S1 (E484K), (K417N), (N501Y) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (D614G) protein (His-Avi Tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (BA.4 - Omicron), (BA.5 - Omicron) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (BA.2.12.1 - Omicron) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
SARS-CoV-2 Spike S1 (B.1.617.2 - delta) protein (His tag)宿主: SARS Coronavirus-2 (SARS-CoV-2) 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
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- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 缓冲液
- PBS, pH 7.4
- 注意事项
- Please avoid repeated freeze-thaw cycles.
- 储存条件
- -20 °C
- 储存方法
- -20°C
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: "Point-of-care (POC) SARS-CoV-2 antigen detection using functionalized aerosol jet-printed organic electrochemical transistors (OECTs)." in: Nanoscale, Vol. 15, Issue 11, pp. 5476-5485, (2023) (PubMed).
: "Delayed production of neutralizing antibodies correlates with fatal COVID-19. ..." in: Nature medicine, (2021) (PubMed).
: "Divergent and self-reactive immune responses in the CNS of COVID-19 patients with neurological symptoms. ..." in: Cell reports. Medicine, pp. 100288, (2021) (PubMed).
: "Validation of high-throughput, semiquantitative solid phase SARS coronavirus-2 serology assays in serum and dried blood spot matrices." in: Bioanalysis, (2021) (PubMed).
: "Comparative analyses of SARS-CoV-2 binding (IgG, IgM, IgA) and neutralizing antibodies from human serum samples." in: Journal of immunological methods, Vol. 489, pp. 112937, (2021) (PubMed).
: "A Combination of Receptor-Binding Domain and N-Terminal Domain Neutralizing Antibodies Limits the Generation of SARS-CoV-2 Spike Neutralization-Escape Mutants. ..." in: mBio, Vol. 12, Issue 5, pp. e0247321, (2021) (PubMed).
: "Immune responses to SARS-CoV-2 infection in hospitalized pediatric and adult patients." in: Science translational medicine, Vol. 12, Issue 564, (2020) (PubMed).
: "Successful transfer of anti-SARS-CoV-2 immunity using convalescent plasma in an MM patient with hypogammaglobulinemia and COVID-19." in: Blood advances, Vol. 4, Issue 19, pp. 4864-4868, (2020) (PubMed).
: "HDL-scavenger receptor B type 1 facilitates SARS-CoV-2 entry." in: Nature metabolism, Vol. 2, Issue 12, pp. 1391-1400, (2020) (PubMed).
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: "Point-of-care (POC) SARS-CoV-2 antigen detection using functionalized aerosol jet-printed organic electrochemical transistors (OECTs)." in: Nanoscale, Vol. 15, Issue 11, pp. 5476-5485, (2023) (PubMed).
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- 抗原
- SARS-CoV-2 Spike S1
- Abstract
- SARS-CoV-2 Spike S1 产品
- 别名
- E2 Protein, Surface Glycoprotein Protein, S Protein
- 物质类
- Viral Protein
- 背景
- It's been reported that Coronavirus can infect the human respiratory epithelial cells through interaction with the human ACE2 receptor. The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
- 分子量
- 76.9 kDa
- 基因ID
- 43740568
- UniProt
- P0DTC2
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