Interleukin 17a 蛋白
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- 抗原 See all Interleukin 17a (IL17A) 蛋白
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- 蛋白类型
- Recombinant
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宿主
- 大鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 012-001-b32
- Supplier
- Rockland
- 原理
- IL-17A Rat Recombinant Protein
- 纯化方法
- Purity was determined to be greater than 97% by reducing and non-reducing SDS- PAGE.
- 纯度
- 97,00%
- 内毒素水平
- Measured by LAL is 0.03 EU/μg protein.
- Biological Activity Comment
- Rat IL-17A is fully biologically active when compared to standards via dose-dependent induction of IL-6 production in cultured mouse NIH 3T3 fibroblasts. The ED50 for recombinant rat IL-17A in this assay was 0.44-0.66ng/ml.
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- Discover our top product IL17A 蛋白
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Interleukin 17A (IL17A) (AA 24-155) protein (His tag)
IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Interleukin 17A (IL17A) (AA 24-155) protein (His-Avi Tag,Biotin)IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (AA 24-155) protein (His-Avi Tag)IL17A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 92 % as determined by HPLC
Interleukin 17A (IL17A) (AA 26-158) protein (His-Avi Tag,Biotin)IL17A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (Active) protein (His tag)IL17A 宿主: 小鼠 宿主: 人细胞 Recombinant > 95 % as determined by SDS-PAGE Active
Interleukin 17A (IL17A) (Active) protein (His tag)IL17A 宿主: Cynomolgus 宿主: 人细胞 Recombinant >95 % as determined by reducing SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 26-158) protein (His-Avi Tag)IL17A 宿主: 小鼠 宿主: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His tag)IL17A 宿主: Cynomolgus 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 24-155) (Active) protein (His tag,AVI tag,Biotin)IL17A 宿主: 人 宿主: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Interleukin 17A (IL17A) (AA 26-158) protein (His tag)IL17A 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- 应用备注
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Other: User Optimized
Application_Note: IL-17A protein has been tested by SDS-PAGE and is suitable as a control for polyclonal or monoclonal anti-IL-17A in immunological assays.
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 250 μL
- 浓度
- 0.1 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Citrate, pH 3.0
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL) . For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- Interleukin 17a (IL17A) (Interleukin 17A (IL17A))
- 别名
- Il17a (IL17A 产品)
- 背景
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Synonyms: Interleukin-17A cytokine, IL-17A, IL-17, Cytotoxic T-lymphocyte-associated antigen 8, CTLA-8
Background: Rat IL- 17A (IL-17), also known as CTLA-8, is a proinflammatory cytokine member of a six-species family of proteins (IL-17A-17F). Rat IL-17A protein is a homodimer consisting of two 134 amino acids peptides. IL-17A is secreted mainly by activated CD4+ and CD8+ T lymphocytes and acts through its receptor, IL-17R, to induce the expression of many mediators of inflammation, most strikingly, those that are involved in the proliferation, maturation and chemotaxis of neutrophils. Elevated levels of IL-17A have been associated with several conditions, including rheumatoid arthritis, airway inflammation, allograft rejection, inflammatory bowel disease, psoriasis, cancer and multiple sclerosis. There is 58 % identity between the amino acid sequence of human and rat IL-17A. Recombinant rat IL-17A produced in E.coli is a non-glycosylated disulfide-joined homodimer having a molecular mass of 30.0 kDa.
- UniProt
- Q61453
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