VEGFA 蛋白
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- 抗原 See all VEGFA 蛋白
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-b99-0010
- Supplier
- Rockland
- 原理
- Mouse Vascular Endothelial Growth Factor-165 Recombinant Protein
- 纯化方法
- Vascular Endothelial Growth Factor-165 purity was determined to be greater than 95% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- 纯度
- 95,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of human umbilical vein endothelial cells (HUVEC) and is typically 1-5 ng/mL.
- Top Product
- Discover our top product VEGFA 蛋白
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Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)
VEGFA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (Fc Tag)VEGFA 宿主: 人 宿主: HEK-293 Cells Recombinant The purity of the protein is greater than 95 % as determined by SDS-PAGE and Coomassie blue staining.
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (His tag,AVI tag,Biotin)VEGFA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Func Active
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-146) (Active) protein (His tag,AVI tag,Biotin)VEGFA 宿主: 小鼠 宿主: HEK-293 Cells Recombinant >95 % as determined by SDS-PAGE. Active
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-147) protein (His tag)VEGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Vascular Endothelial Growth Factor A (VEGFA) (AA 1-147) protein (GST tag)VEGFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA 宿主: 大鼠 宿主: 酵母菌 Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) proteinVEGFA 宿主: 小鼠 宿主: Yeast (Pichia pastoris) Recombinant > 95 % as determined by SDS-PAGE
Vascular Endothelial Growth Factor A (VEGFA) (AA 27-190) (Active) protein (His tag)VEGFA 宿主: 大鼠 宿主: HEK-293 Cells > 97 % AcA, CC Active
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- 应用备注
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Other: User Optimized
Application_Note: Endothelial Growth Factor-165 Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Endothelial Growth Factor-165 in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- VEGFA (Vascular Endothelial Growth Factor A (VEGFA))
- 别名
- Vegfa (VEGFA 产品)
- 背景
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Synonyms: VEGF-A, glioma-derived endothelial cell mitogen, Vascular permeability factor (VPF)
Background: Vascular Endothelial Growth Factor-A (VEGF-A) was originally isolated from tumor cells and is produced by a wide variety of cell types. In addition to stimulating vascular growth and vascular permeability, VEGF-A may play a role in stimulating vasodilation via nitric oxide-dependent pathways. VEGF-A has several variants, VEGF-165 being the most abundant. Rat and bovine VEGF are one amino acid shorter than the human factor, and the bovine and human sequences show a homology of 95 % . Recombinant mouse VEGF-165 is a non-glycosylated, disulfide-linked homodimer, containing 165 amino acids, with a molecular weight of 39 kDa.
- UniProt
- Q00731-2
- 途径
- RTK signaling, Glycosaminoglycan Metabolic Process, Regulation of Cell Size, Tube Formation, Signaling Events mediated by VEGFR1 and VEGFR2, Platelet-derived growth Factor Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling
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