14-3-3 zeta Protein (YWHAZ) (GST tag)
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- 抗原 See all 14-3-3 zeta (YWHAZ) 蛋白
- 14-3-3 zeta (YWHAZ)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This 14-3-3 zeta protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r27s
- Supplier
- Rockland
- 原理
- 14-3-3 zeta recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human tag-free 14-3-3ζ was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein . The purity was determined to be >90% by densitometry.
- 纯度
- >90%
- Top Product
- Discover our top product YWHAZ 蛋白
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14-3-3 zeta (YWHAZ) (AA 1-245) protein (GST tag)
YWHAZ 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
14-3-3 zeta (YWHAZ) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)YWHAZ 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
14-3-3 zeta (YWHAZ) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)YWHAZ 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: 14-3-3ζ Protein is suitable for use in Western Blot. Expect a band approximately ~ 55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- 14-3-3ζ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- 14-3-3 zeta (YWHAZ)
- 别名
- YWHAZ (YWHAZ 产品)
- 背景
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Synonyms: 14-3-3 zeta, YWHAZ, KCIP-1, MGC111427, MGC126532, MGC138156, 14-3-3 protein zeta, 14-3-3-like protein, Protein Leonardo, 14-3-3, 14-3-3EZ, leo, THAP
Background: 14-3-3ζ (also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, zeta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. 14-3-3ζ protein plays a key role in cancer biology by being an important regulator of major cellular processes such as proliferation, differentiation, senescence and apoptosis (1). 14-3-3ζ protein has been shown to interact with the IRS1 protein, suggesting a role for this protein in regulating insulin sensitivity by interrupting the association between the insulin receptor and IRS1 (2). 14-3-3ζ Protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, WNT Signaling research.
- NCBI登录号
- NM_003406
- 途径
- Apoptosis, Hormone Transport, Myometrial Relaxation and Contraction, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Synaptic Membrane, Production of Molecular Mediator of Immune Response, Maintenance of Protein Location
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