14-3-3 theta Protein (YWHAQ)
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- 抗原 See all 14-3-3 theta (YWHAQ) 蛋白
- 14-3-3 theta (YWHAQ)
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r30s
- Supplier
- Rockland
- 原理
- 14-3-3 theta recombinant protein
- 纯化方法
- Recombinant full-length human 14-3-3θ was expressed in E. coli cells. The purity was determined to be >90% by densitometry.
- 纯度
- >90%
- Top Product
- Discover our top product YWHAQ 蛋白
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: 14-3-3θ Protein is suitable for use in Western Blot. Expect a band approximately ~ 29 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- 14-3-3θ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- 14-3-3 theta (YWHAQ)
- 别名
- YWHAQ (YWHAQ 产品)
- 背景
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Synonyms: 14-3-3 theta, YWHAQ, 1C5, HS1, 14-3-3, 14-3-3 protein tau
Background: 14-3-3θ (also known as tyrosine 3-monooxygenase/ tryptophan 5-monooxygenase activation protein, theta polypeptide) is a member of the 14-3-3 family of proteins which mediate signal transduction by binding to phosphoserine-containing proteins. Through interaction with ASK1, c-jun NH-terminal kinase, and p38 mitogen-activated protein kinase (MAPK), 14-3-3θ plays an important role in controlling apoptosis (1). Induced expression of 14-3-3θ protein has been reported in patients with amyotrophic lateral sclerosis. Additionally, 14-3-3θ has been observed to mediate nucleocytoplasmic shuttling of the N protein (coronavirus nucleocapsid protein) which causes severe acute respiratory syndrome (2). 14-3-3θ Protein is ideal for investigators involved in Cell Stress& Chaperone Proteins, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.
- NCBI登录号
- NM_006142
- 途径
- Apoptosis, Myometrial Relaxation and Contraction
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