YWHAE Protein (GST tag)
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- 抗原 See all YWHAE 蛋白
- YWHAE (14-3-3 epsilon (YWHAE))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This YWHAE protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r25s
- Supplier
- Rockland
- 原理
- 14-3-3 epsilon recombinant protein-GST fusion protein
- 纯化方法
- Recombinant full-length human 14-3-3ε was expressed in E.coli cells using an N-terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >90% by densitometry.
- 纯度
- >90%
- Top Product
- Discover our top product YWHAE 蛋白
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14-3-3 epsilon (YWHAE) (AA 1-255) protein (His tag)
YWHAE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
14-3-3 epsilon (YWHAE) (AA 1-255) protein (GST tag)YWHAE 宿主: 人 宿主: 小麦胚 Recombinant ELISA, WB, AA, AP
14-3-3 epsilon (YWHAE) protein (GST tag)YWHAE 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % as determined by SDS-PAGE
14-3-3 epsilon (YWHAE) (AA 1-255) proteinYWHAE 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % pure SDS
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: 14-3-3ε Protein is suitable for use in Western Blot. Expect a band approximately ~55 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- 14-3-3ε Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- YWHAE (14-3-3 epsilon (YWHAE))
- 别名
- YWHAE (YWHAE 产品)
- 背景
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Synonyms: 14-3-3 epsilon, YWHAE, MDS, MDCR, KCIP-1, 14-3-3E, FLJ45465
Background: 14-3-3ε also known as tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein is a member of the 14-3-3 family. 14-3-3ε has been reported to be involved in heat shock transcription factor 1 (HSF1) and extracellular signal regulated protein kinase (ERK) pathways (1). 14-3-3ε interaction with the deubiquitinating enzyme UBPY, also known as USP8, regulates cargo sorting and membrane traffic at early endosomes. Association of 14-3-3ε, HSF1 with ERK during heat shock may modulate the amplitude of the response and control the termination of HSP expression on resumption of growth conditions (2). 14-3-3ε protein is ideal for investigators involved in Cell Stress & Chaperone Proteins, Cell Signaling, Cancer research, Cell Cycle, Cellular Stress, Neurobiology, and WNT Signaling research.
- NCBI登录号
- NM_006761
- 途径
- Neurotrophin Signaling Pathway, Myometrial Relaxation and Contraction, M Phase
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