CXCL12 蛋白
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- 抗原 See all CXCL12 蛋白
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- 大肠杆菌(E. Coli)
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-w23-0010
- Supplier
- Rockland
- 原理
- Mouse Stromal Cell-Derived Factor-1 beta (CXCL12) Recombinant Protein
- 纯化方法
- Stromal Cell-Derived Factor-1 beta (CXCL12) purity was determined to be greater than 97% as determined by HpLC, analysis by UV-Spectroscopy at 280nm, and by reducing and non-reducing SDS-pAGE.
- 纯度
- 97,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the ability to chemoattract human T cells at 10-75 ng/mL.
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- Discover our top product CXCL12 蛋白
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- 应用备注
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Other: User Optimized
Application_Note: Stromal Cell-Derived Factor-1 beta Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Stromal Cell-Derived Factor-1 beta in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- CXCL12 (Chemokine (C-X-C Motif) Ligand 12 (CXCL12))
- 别名
- Cxcl12 (CXCL12 产品)
- 背景
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Synonyms: 12-O-tetradecanoylphorbol 13-acetate repressed protein 1 (TPAR1), C-X-C motif chemokine 12, Pre-B cell growth-stimulating factor (PBSF), Thymic lymphoma cell-stimulating factor (TLSF)
Background: Stromal Derived Growth Factor-1β (SDF-1β), also called CXCL12, is one of two splice variants made by a wide variety of cells when stimulated by inflammatory cytokines such as, TNF, IL-1 or LPS. SDF-1β signals through the G protein-couple receptor, CXCR4, to recruit activated leukocytes. Human SDF-1β shares 100 % identity to feline SDF-1β. Recombinant mouse SDF-1β is a non-glycosylated protein, containing 72 amino acids, with a molecular weight of 8.5 kDa.
- UniProt
- Q4FJL5
- 途径
- Regulation of Cell Size, CXCR4-mediated Signaling Events, Negative Regulation of intrinsic apoptotic Signaling
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