14-3-3 sigma/SFN 蛋白
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- 抗原 See all 14-3-3 sigma/SFN (SFN) 蛋白
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-r32s
- Supplier
- Rockland
- 原理
- 14-3-3 sigma recombinant protein
- 纯化方法
- Recombinant full-length human 14-3-3σ was expressed in E. coli cells. The purity was determined to be >95% by densitometry.
- 纯度
- >95%
- Top Product
- Discover our top product SFN 蛋白
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Stratifin (SFN) (AA 1-248) protein (GST tag)
SFN 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Stratifin (SFN) (AA 1-248), (full length) protein (GST tag)SFN 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % SDS
Stratifin (SFN) proteinSFN 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % SDS, WB, ELISA, IP
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: 14-3-3σ Protein is suitable for use in Western Blot. Expect a band approximately ~ 29 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- 14-3-3σ Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- 14-3-3 sigma/SFN (SFN) (Stratifin (SFN))
- 别名
- YWHAS (SFN 产品)
- 背景
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Synonyms: 14-3-3 sigma, SFN, stratifin, YWHAS
Background: 14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents (1). The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest (2). Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death. 14-3-3σ Protein is ideal for investigators involved in Cell Stress& Chaperone Proteins, AKT/PKB Pathway, Cancer, Cell Cycle, Cellular Stress, ERK/MAPK Pathway, Neurobiology, PKA/PKC Pathway, and WNT Signaling research.
- NCBI登录号
- NM_006826
- 途径
- p53 Pathway, Myometrial Relaxation and Contraction
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