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Sonic Hedgehog Protein (SHH)

Rockland 009-001-w24-0005 SHH 宿主: 人 宿主: 大肠杆菌(E. Coli) Recombinant 97,00% SDS
Rockland
产品编号 ABIN6700556
Supplier Product No.: 009-001-w24-0005
发货至: 中国
  • 抗原 See all Sonic Hedgehog (SHH) 蛋白
    Sonic Hedgehog (SHH)
    蛋白类型
    Recombinant
    宿主
    • 14
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    资源
    • 20
    • 18
    • 5
    • 1
    • 1
    大肠杆菌(E. Coli)
    应用范围
    SDS-PAGE (SDS)
    Supplier Product No.
    009-001-w24-0005
    Supplier
    Rockland
    原理
    Human Sonic Hedgehog Recombinant Protein
    纯化方法
    Sonic Hedgehog purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    纯度
    97,00%
    内毒素水平
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is measured by the dose-dependent induction of alkaline phosphatase production by C3H/10T1/2 (CCL-226) fibroblasts and is typically 0.8-1 μg/mL.
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    Discover our top product SHH 蛋白
  • 应用备注

    Other: User Optimized

    Application_Note: Sonic Hedgehog Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Sonic Hedgehog in immunological assays.

    说明

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 5 μL (5-50 μL)

    缓冲液

    Buffer: 0.01 M Sodium Phosphate, pH 7.5

    Stabilizer: None

    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    有效期
    6 months
  • 抗原
    Sonic Hedgehog (SHH)
    别名
    SHH (SHH 产品)
    背景

    Synonyms: HHG-1

    Background: Sonic hedgehog (SHH) is a member of a small group of secreted proteins that are essential for development in both vertebrates and invertebrates. Three mammalian hedgehog genes (sonic, desert, Indian) share about 60 % homology and all signal via the same receptors. Recombinant human SHH is a non-glycosylated protein, containing 175 amino acids, with a molecular weight of 19.7 kDa. The Cys at position 25 has been substituted with Ile.

    UniProt
    Q15465
    途径
    Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development
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