SIRT1 Protein (GST tag)
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- 抗原 See all SIRT1 蛋白
- SIRT1 (Sirtuin 1 (SIRT1))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 标记
- This SIRT1 protein is labelled with GST tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-t31s
- Supplier
- Rockland
- 原理
- SIRT1 recombinant protein-GST fusion protein
- 纯化方法
- Recombinant human SIRT1 (193-end) was expressed in E. coli cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >80% by densitometry.
- 纯度
- >80%
- Top Product
- Discover our top product SIRT1 蛋白
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Sirtuin 1 (SIRT1) (AA 510-747) protein (His tag)
SIRT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Sirtuin 1 (SIRT1) (AA 456-555) protein (GST tag)SIRT1 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Sirtuin 1 (SIRT1) (AA 254-495) protein (His tag)SIRT1 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant > 90 % by SDS - PAGE SDS
Sirtuin 1 (SIRT1) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)SIRT1 宿主: 人 宿主: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: SIRT1 Protein is suitable for use in Western Blot. Expect a band approximately ~105 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.2 μg/μL
- 缓冲液
- SIRT1 Protein is stored in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- SIRT1 (Sirtuin 1 (SIRT1))
- 别名
- SIRT1 (SIRT1 产品)
- 背景
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Synonyms: SIR2L1, Sirtuin 1,NAD-dependent protein deacetylase sirtuin-1, Regulatory protein SIR2 homolog 1, SIR2-like protein 1, SirtT1 75 kDa fragment, 75SirT1
Background: SIRT1 is a member of the sirtuin family of proteins which are homologs to the yeast Sir2 protein. Sirtuin family contain a sirtuin core domain and are grouped into four classes with SIRT1 being a member of class I. SIRT1 is a stress-response and chromatin-silencing factor (1). It is an NAD(+)-dependent histone deacetylase involved in various nuclear events such as transcription, DNA replication, and DNA repair. SIRT1 protein binds and deacetylates the p53 protein (2). Expression of wild type SIRT1 in human cells reduces the transcriptional activity of p53 indicating that SIRT1 is involved in the regulation of p53 function via deacetylation. SIRT1 Protein is ideal for investigators involved in Signaling Proteins, Deacetylase/Demethylase Proteins, Cancer, Cell Cycle, and Inflammation research.
- NCBI登录号
- NM_012238
- 途径
- MAPK Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Carbohydrate Homeostasis, Positive Regulation of Endopeptidase Activity, Regulation of Carbohydrate Metabolic Process, Positive Regulation of Response to DNA Damage Stimulus, Negative Regulation of intrinsic apoptotic Signaling
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