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SCYL3 Protein (GST tag)

Rockland 009-001-t27s SCYL3 宿主: 人 宿主: Insect cells (Sf9) Recombinant >85% WB
Rockland
产品编号 ABIN6700491
Supplier Product No.: 009-001-t27s
发货至: 中国
  • 抗原 See all SCYL3 蛋白
    SCYL3 (SCY1-Like 3 (SCYL3))
    蛋白类型
    Recombinant
    宿主
    资源
    • 1
    • 1
    Insect cells (Sf9)
    标记
    This SCYL3 protein is labelled with GST tag.
    应用范围
    Western Blotting (WB)
    Supplier Product No.
    009-001-t27s
    Supplier
    Rockland
    原理
    SCYL3 recombinant protein-GST fusion protein
    纯化方法
    Recombinant human SCYL3 (2-end) was expressed by baculovirus in Sf9 insect cells using an N-Terminal Glutathione-S-Transferase fusion protein. The purity was determined to be >85% by densitometry.
    纯度
    >85%
    Top Product
    Discover our top product SCYL3 蛋白
  • 应用备注

    Western_Blot_Dilution: User Optimized

    Application_Note: SCYL3 Protein is suitable for use in Western Blot. Expect a band approximately ~110 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.

    限制
    仅限研究用
  • 状态
    Liquid
    浓度
    0.1 μg/μL
    缓冲液
    SCYL3 Protein is stored in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl, 10 mM glutathione, 0.1 mM EDTA, 0.25 mM DTT, 0.1 mM PMSF, 25 % glycerol.
    储存条件
    -80 °C
    储存方法
    Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
    有效期
    12 months
  • 抗原
    SCYL3 (SCY1-Like 3 (SCYL3))
    别名
    SCYL3 (SCYL3 产品)
    背景

    Synonyms: PACE-1, PACE1, RP1-97P20.2, Protein-associating with the carboxyl-terminal domain of ezrin, Ezrin-binding protein PACE-1, SCY1-like protein 3, SCYL3

    Background: SCYL3 encodes a protein with a kinase domain and four HEAT repeats which interact with the C-terminal domain of ezrin, an ERM protein which play a role in cell adhesion and migration (1). C-terminal domain of SCYL3 interacts with the C-terminal alpha-helical domain of ezrin. Biochemical assays indicate that SCYL3 had associated rather than intrinsic protein kinase activity. SCYL3 is associated with the cytoplasmic face of the Golgi apparatus. This distribution is dependent upon the presence of the SCYL3 N-terminal myristoylation consensus sequence but is not dependent on an association with ezrin. SCYL3 is highly expressed in breast carcinoma cells and adult colon. SCYL3 Protein is ideal for investigators involved in Signaling Proteins, Cellular Proteins, Inflammation, Invasion/Metastasis, and Neurobiology research.

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