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- 抗原 See all PDGFB 蛋白
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- Supplier Product No.
- 010-001-w12-0010
- Supplier
- Rockland
- 原理
- Mouse Platelet Derived Growth Factor BB Recombinant Protein
- 纯化方法
- platelet Derived Growth Factor BB purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- 纯度
- 98,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is measured by the dose-dependent proliferation in mouse 3T3 fibroblasts and is typically 1-2 ng/mL.
- Top Product
- Discover our top product PDGFB 蛋白
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Platelet Derived Growth Factor Subunit B (PDGFB) (Active) protein
PDGFB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) protein (GST tag)PDGFB 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) protein (His tag)PDGFB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Platelet Derived Growth Factor Subunit B (PDGFB) (AA 82-190) (Active) protein (His tag,AVI tag)PDGFB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE. Active
Platelet Derived Growth Factor Subunit B (PDGFB) proteinPDGFB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
Platelet Derived Growth Factor Subunit B (PDGFB) proteinPDGFB 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant 95,00% SDS
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- 应用备注
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Other: User Optimized
Application_Note: Platelet Derived Growth Factor-BB Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-BB in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 浓度
- 0.1 mg/mL
- 缓冲液
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Buffer: 0.01 M Sodium Citrate, pH 3.0
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
- 别名
- Pdgfb (PDGFB 产品)
- 背景
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Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis
Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant mouse PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acids chains, with a total molecular weight of 24.6 kDa.
- UniProt
- P31240
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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