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PDGFB 蛋白

Rockland 010-001-w12-0010 PDGFB 宿主: 小鼠 宿主: 大肠杆菌(E. Coli) Recombinant 98,00%
Rockland
产品编号 ABIN6700408
Supplier Product No.: 010-001-w12-0010
发货至: 中国
  • 抗原 See all PDGFB 蛋白
    PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
    蛋白类型
    Recombinant
    宿主
    • 11
    • 3
    • 3
    • 2
    • 1
    • 1
    • 1
    小鼠
    资源
    • 18
    • 3
    • 1
    大肠杆菌(E. Coli)
    Supplier Product No.
    010-001-w12-0010
    Supplier
    Rockland
    原理
    Mouse Platelet Derived Growth Factor BB Recombinant Protein
    纯化方法
    platelet Derived Growth Factor BB purity was determined to be greater than 98% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
    纯度
    98,00%
    内毒素水平
    Measured by LAL is typically ≤ 1 EU/μg protein.
    Biological Activity Comment
    The activity is measured by the dose-dependent proliferation in mouse 3T3 fibroblasts and is typically 1-2 ng/mL.
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    Discover our top product PDGFB 蛋白
  • 应用备注

    Other: User Optimized

    Application_Note: Platelet Derived Growth Factor-BB Recombinant Protein has been tested by biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-BB in immunological assays.

    说明

    Suggested_Applications: Cellular Assay

    Other_Performance_Data:

    限制
    仅限研究用
  • 状态
    Lyophilized
    溶解方式

    Reconstitution_Buffer: Restore with deionized water (or equivalent)

    Reconstitution_Volume: 10 μL (10-100 μL)

    浓度
    0.1 mg/mL
    缓冲液

    Buffer: 0.01 M Sodium Citrate, pH 3.0

    Stabilizer: None

    储存液
    Without preservative
    储存条件
    4 °C,-20 °C
    储存方法
    Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
    有效期
    6 months
  • 抗原
    PDGFB (Platelet Derived Growth Factor Subunit B (PDGFB))
    别名
    Pdgfb (PDGFB 产品)
    背景

    Synonyms: PDGF-2, Platelet-derived growth factor B chain, Platelet-derived growth factor beta polypeptide, Proto-oncogene c-Sis

    Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant mouse PDGF-BB is a non-glycosylated, disulfide-linked homodimer, containing two 109 amino acids chains, with a total molecular weight of 24.6 kDa.

    UniProt
    P31240
    途径
    RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Regulation of Carbohydrate Metabolic Process, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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