PDGFA 蛋白
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- 抗原 See all PDGFA 蛋白
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- 蛋白类型
- Recombinant
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宿主
- 小鼠
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 010-001-w08-0010
- Supplier
- Rockland
- 原理
- Mouse Platelet Derived Growth Factor AA Recombinant Protein
- 纯化方法
- platelet Derived Growth Factor AA purity was determined to be greater than 97% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- 纯度
- 97,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by the dose-dependent proliferation of Balb/c 3T3 cells and is typically 8-10 ng/mL.
- Top Product
- Discover our top product PDGFA 蛋白
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Platelet Derived Growth Factor A (PDGFA) (Active) protein
PDGFA 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant >95 % as determined by SDS-PAGE Func Active
Platelet Derived Growth Factor A (PDGFA) (AA 1-211) protein (GST tag)PDGFA 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AA, AP
Platelet Derived Growth Factor A (PDGFA) (AA 94-194) (Active) protein (His tag)PDGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） > 95 % AcA, CC Active
Platelet Derived Growth Factor A (PDGFA) (Active) proteinPDGFA 宿主: 小鼠 宿主: 大肠杆菌（E. Coli） Recombinant > 95 % as determined by SDS-PAGE and Coomassie blue staining Func, AbP, STD, PI Active
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- 应用备注
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Other: User Optimized
Application_Note: Platelet Derived Growth Factor-AA Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Platelet Derived Growth Factor-AA in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: Restore with deionized water (or equivalent)
Reconstitution_Volume: 10 μL (10-100 μL)
- 缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- PDGFA (Platelet Derived Growth Factor A (PDGFA))
- 别名
- Pdgfa (PDGFA 产品)
- 背景
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Synonyms: PDGF-1, Platelet-derived growth factor A chain, Platelet-derived growth factor alpha polypeptide
Background: Platelet-Derived Growth Factor (PDGF) is a mitogenic peptide growth hormone carried in the alpha-granules of platelets and is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication. The synthesis of PDGF can be induced by IL-1, IL-6, TNF-α, TGF-β and EGF. Recombinant mouse PDGF-AA is a non-glycosylated, disulfide-linked homodimer, containing two 126 amino acid chains, with a total molecular weight of 28.9 kDa.
- UniProt
- P20033
- 途径
- RTK signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Smooth Muscle Cell Migration, Platelet-derived growth Factor Receptor Signaling
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