MGMT Protein (His tag)
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- 抗原 See all MGMT 蛋白
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- 蛋白类型
- Recombinant
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宿主
- 人
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资源
- Insect cells (Sf9)
- 标记
- This MGMT protein is labelled with His tag.
- 应用范围
- Western Blotting (WB)
- Supplier Product No.
- 009-001-s47s
- Supplier
- Rockland
- 原理
- MGMT recombinant protein-HIS Epitope
- 纯化方法
- Recombinant full length human MGMT was expressed by baculovirus in Sf9 insect cells using an N-Terminal his epitope. The purity was determined to be >95% by densitometry.
- 纯度
- >95%
- Top Product
- Discover our top product MGMT 蛋白
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O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (His-IF2DI Tag)
MGMT 宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
O6-Methylguanine-DNA-Methyltransferase (MGMT) (AA 1-207) protein (GST tag)MGMT 宿主: 人 宿主: 小麦胚 Recombinant WB, ELISA, AP, AA
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- 应用备注
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Western_Blot_Dilution: User Optimized
Application_Note: MGMT Protein is suitable for use in Western Blot. Expect a band approximately ~25 kDa on specific lysates or tissues. Specific conditions for reactivity should be optimized by the end user.
- 限制
- 仅限研究用
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- 状态
- Liquid
- 浓度
- 0.1 μg/μL
- 缓冲液
- MGMT Protein is stored in 50 mM sodium phosphate, pH 7.0, 300 mM NaCl, 150 mM imidazole, 0.1 mM PMSF, 0.25 mM DTT, 25 % glycerol.
- 储存条件
- -80 °C
- 储存方法
- Store product at -70°C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
- 有效期
- 12 months
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- 抗原
- MGMT (O6-Methylguanine-DNA-Methyltransferase (MGMT))
- 别名
- MGMT (MGMT 产品)
- 背景
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Synonyms: Methylated-DNA--protein-cysteine methyltransferase, EC 2.1.1.63, 6-O-methylguanine-DNA methyltransferase, MGMT, O-6-methylguanine-DNA-alkyltransferase
Background: MGMT or O-6-methylguanine-DNA methyltransferase is the major mutagenic and carcinogenic lesion in DNA induced by simple alkylating mutagens. The methylation of the MGMT promoter in gliomas is a useful predictor of the responsiveness of the tumors to alkylating agents (1). The clinical trial substantiates the predictive value of O-6-methylguanine-DNA methyltransferase promoter methylation in glioblastoma patients treated with temozolomide (2). Methylation of the MGMT promoter in tumors is associated with longer survival in glioblastoma patients. MGMT Protein is ideal for investigators involved in Signaling Proteins, Acetyl/Methyltransferase Proteins, Apoptosis/Autophagy, Cancer, Cell Cycle, and Neurobiology research.
- 途径
- DNA Damage Repair, Positive Regulation of Response to DNA Damage Stimulus
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