Myostatin Propeptide 蛋白
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- 抗原
- Myostatin Propeptide
- 蛋白类型
- Recombinant
- 宿主
- 人
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资源
- 大肠杆菌（E. Coli）
- 应用范围
- SDS-PAGE (SDS)
- Supplier Product No.
- 009-001-w02-0025
- Supplier
- Rockland
- 原理
- Human Myostatin Propeptide Recombinant Protein
- 纯化方法
- Myostatin propeptide purity was determined to be greater than 95% as determined by analysis by UV-Spectroscopy at 280nm and by reducing and non-reducing SDS-pAGE.
- 纯度
- 95,00%
- 内毒素水平
- Measured by LAL is typically ≤ 1 EU/μg protein.
- Biological Activity Comment
- The activity is determined by its ability to inhibit 50 ng/mL of Myostatin on MPC-11 cells and is typically 0.01-0.04 μg/mL.
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Myostatin Propeptide (AA 23-266) protein (His tag)
宿主: 人 宿主: 大肠杆菌（E. Coli） Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
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- 应用备注
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Other: User Optimized
Application_Note: Myostatin Propeptide Recombinant Protein has been tested by SDS-PAGE and biological activity and is suitable as a control for polyclonal or monoclonal anti-Myostatin Propeptide in immunological assays.
- 说明
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Suggested_Applications: Cellular Assay
Other_Performance_Data:
- 限制
- 仅限研究用
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- 状态
- Lyophilized
- 溶解方式
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Reconstitution_Buffer: 0.02M HCl
Reconstitution_Volume: 25 μL (25-250 μL)
- 浓度
- 0.1 mg/mL
- 缓冲液
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Buffer: 0.1 % Trifluoroacetic acid
Stabilizer: None
- 储存液
- Without preservative
- 储存条件
- 4 °C,-20 °C
- 储存方法
- Store vial at 4° C prior to restoration. Dilute only prior to immediate use. Maintain sterility. This product DOES NOT contain preservative. DO NOT VORTEX. We recommend adding a carrier protein such as HSA or BSA to 0.1% (i.e. 1.0 mg/mL). For best results aliquot contents and freeze at -20° C or colder. Avoid cycles of freezing and thawing. Centrifuge vial before each opening to dislodge contents from the cap and to clarify if contents are not clear after standing at room temperature.
- 有效期
- 6 months
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- 抗原
- Myostatin Propeptide
- 背景
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Synonyms: Myostatin
Background: Myostatin (GDF-8), a member of the TGF-β superfamily, is a potent and specific negative regulator of skeletal muscle mass. The myostatin propeptide is known to bind and inhibit myostatin in vitro. This interaction is relevant in vivo, with a majority (>70 % ) of myostatin in serum bound to its propeptide acting as a negative regulator of myostatin. Recombinant human Myostatin Propeptide is a non-glycosylated protein, containing 244 amino acids, with a molecular weight of 27.8 kDa.
- UniProt
- O08689
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